不明原因复发性流产患者血浆中PAI-1水平表达及其基因启动区4G/5G位点多态性分析  

Expresssion of Level of Plasma PAI-1 in Patients with Unexplained Recurrent Spontaneous Abortion and Polymorphism Analysis of 4G/5G Loci in Its Gene Promoter Region

作　　者：刘玉美 胡春青 唐晓勇 雷永革 李春容 蓝红 LIU Yumei;HU Chunqing;TANG Xiaoyong;LEI Yongge;LI Chunrong;LAN Hong(Shenzhen Dapeng New District Maternal and Child Health Hospital,Shenzhen 518120,China;不详)

机构地区：[1]深圳市大鹏新区妇幼保健院,广东深圳518120 [2]中国科学院大学深圳医院(光明)

出　　处：《中外医学研究》2022年第10期81-84,共4页CHINESE AND FOREIGN MEDICAL RESEARCH

基　　金：深圳市大鹏新区科技创新和经济服务局基金项目(YL202001-11)。

摘　　要：目的:探讨不明原因复发性流产(unexplained recurrent spontaneous abortion,URSA)患者血浆纤溶酶原激活物抑制剂-1(plasminogen activator inhibitor-1,PAI-1)水平及其基因启动区4G/5G位点单核苷酸多态性(SNP)。方法:选取2019年5月-2021年3月深圳市大鹏新区妇幼保健院妇产科门诊及住院部就诊的URSA患者75例为URSA组,选择同期在本院妇产科门诊及住院部生育史正常的健康育龄妇女120例为对照组。检测两组PAI-1水平,同时检测PAI-1基因启动区4G/5G位点SNP。两组均进行Hardy-Weinberg平衡检验,比较两组PAI-1水平,PAI-1基因启动子区4G/5G SNP,不同基因型URSA患者PAI-1水平。结果:URSA组PAI-1水平为(9.76±2.85)ng/ml,明显高于对照组的(5.23±1.09)ng/ml(t=6.0574,P<0.05)。URSA组PAI-1基因启动子区4G/5G位点的4G/4G基因型和4G等位基因检出率分别为41.33%和64.67%,均明显高于对照组的12.50%和35.00%(P<0.05),且4G/4G基因型和4G等位基因患URSA的相对风险增加(OR=3.7265,95%CI:1.6034,5.9253和OR=2.3825,95%CI:1.3027,3.4801)。携带4G/4G基因型URSA患者PAI-1水平为(12.03±3.17)ng/ml,明显高于5G/4G基因型的(8.52±2.40)ng/ml,而5G/4G基因型患者PAI-1水平又明显高于5G/5G基因型患者的(6.78±1.34)ng/ml,不同基因型患者PAI-1水平比较差异有统计学意义(F=12.3418,P<0.05)。结论:URSA患者PAI-1水平明显升高,同时其基因启动区4G/5G位点呈多态性,且不同基因型URSA患者PAI-1水平存在很大差异,其中4G/4G基因型检出率最高,可能是深圳大鹏新区URSA发病的危险易感基因之一。Objective:To investigate the level of plasminogen activator inhibitor-1(PAI-1)in patients with unexplained recurrent spontaneous abortion(URSA)and single nucleotide polymorphism(SNP)of 4G/5G loci in its gene promoter region.Method:A total of 75 URSA patients treated in the outpatient and inpatient departments of obstetrics and gynecology of Shenzhen Dapeng New District Maternal and Child Health Hospital from May 2019 to March 2021 were selected as the URSA group,and 120 healthy women of childbearing age with normal birth history in the outpatient and inpatient departments of obstetrics and gynecology of our hospital during the same period were selected as the control group.The level of PAI-1 in both groups was detected,and the SNP in 4G/5G loci in the promoter region of PAI-1 gene was detected.Hardy-Weinberg equilibrium test was performed in both groups,the PAI-1 level,4G/5G SNP in the promoter region of PAI-1 gene of the two groups were compared,and PAI-1 level in URSA patients with different genotypes was compared.Result:The level of PAI-1 in URSA group was(9.76±2.85)ng/ml,which was significantly higher than(5.23±1.09)ng/ml in the control group(t=6.0574,P<0.05).The detection rates of 4G/4G genotype and 4G allele at 4G/5G loci in the promoter region of PAI-1 gene in URSA group were 41.33%and 64.67%respectively,which were significantly higher than 12.50%and 35.00%in the control group(P<0.05).The relative risk of URSA increased with 4G/4G genotype and 4G allele(OR=3.7265,95%CI:1.6034,5.9253 and OR=2.3825,95%CI:1.3027,3.4801).PAI-1 level of URSA patients with 4G/4G genotype was(12.03±3.17)ng/ml,which was significantly higher than(8.52±2.40)ng/ml of 5G/4G genotype.The level of PAI-1 in patients with 5G/4G genotype was significantly higher than(6.78±1.34)ng/ml in patients with 5G/5G genotype,and there was significant difference in the level of PAI-1 in patients with different genotypes(F=12.3418,P<0.05).Conclusion:The level of PAI-1 in patients with URSA is significantly increased,and the 4G/5G loci of its gene

关 键 词：不明原因复发性流产 纤溶酶原激活物抑制剂-1 基因 单核苷酸多态性 易感性 

分 类 号：R714.21[医药卫生—妇产科学]