双通道微流控芯片的研制及在RNP抗体和SmD1抗体检测中的应用  

Development of Dual-Channel Microfluidic Chip and Application in Detecting of RNP Antibody and SmD1 Antibody

作　　者：袁榕晨 黄钊 钱纯亘[1,2] 吴力强 汤建新 YUAN Rongchen;HUANG Zhao;QIAN Chungen;WU Liqiang;TANG Jianxin(Hunan Key Laboratory of Biomedical Nanomaterials and Devices,College of Life Science and Chemistry,Hunan University of Technology,Zhuzhou Hunan 412007,China;Shenzhen Yhlo Biotech Co.,Ltd.,Shenzhen Guangdong 518116,China;Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research,Hunan Normal University,Changsha 410081,China)

机构地区：[1]湖南工业大学生命科学与化学学院生物医用纳米材料与器件湖南省重点实验室,湖南株洲412007 [2]深圳市亚辉龙生物科技股份有限公司,广东深圳518116 [3]湖南师范大学化学生物学及中药分析教育部重点实验室,湖南长沙410081

出　　处：《包装学报》2022年第2期22-30,共9页Packaging Journal

基　　金：国家自然科学基金资助项目(51774128);湖南师范大学化学生物学及中药分析教育部重点实验室开放基金资助课题。

摘　　要：自身免疫性疾病发病机制复杂,治愈难度大,因而早期诊断对疾病的有效防治具有重要的意义。为实现从单通道单标志物的单一检测到双通道双标志物的同时检测,开发了一种具有加样孔、储存腔、特斯拉阀、混合区、反应腔、废液腔和流道的双通道微流控芯片。将检测抗体储存于储存腔,利用特斯拉阀控制液体在芯片中的流向。用自制芯片对RNP抗体和SmD1抗体进行检测,检测范围分别为9.6~444.0 AU/mL和12.5~424.8 AU/mL,RSD分别为4.4%和3.5%,检测限分别为5 AU/mL和6.25 AU/mL,检测特异性、重复性良好,可应用于自身免疫性疾病的快速便捷诊断。The pathogenesis of autoimmune diseases is complex and the treatment is difficult,so early diagnosis is of great significance for the effective prevention and treatment of the disease.By storing the detection antibody in the chip,and using the Tesla valve to control the flow direction of the liquid in the chip,a dual-channel microfluidic chip with injection wells,storage chambers,Tesla valves,mixing zones,reaction chambers,waste chambers,and flow paths was developed to realize the detecting model from the single detection of single-marker in one-channel to simultaneous detection of dual-marker in dual-channel on the microfluidic platform.The homemade chip was used to detect RNP antibody and SmD1 antibody items,the detection range was 9.6~444.0 AU/mL for RNP antibody and 12.5~424.8 AU/mL for SmD1 antibody,with the RSD of 4.4%for RNP antibody and 3.5%for SmD1 antibody,the detection limits were 5 AU/mL and 6.25 AU/mL,respectively,and the test results showed good specificity and reproducibility,which can be applied to the rapid and convenient diagnosis of autoimmune diseases.

关 键 词：自身免疫性疾病 双通道微流控芯片 混合结缔组织病 系统性红斑狼疮 特斯拉阀 

分 类 号：R446.62[医药卫生—诊断学]