氯化铯处理对菠菜叶片光系统Ⅱ活性的影响  

Effect of cesium chloride treatment on photosystem Ⅱ activity of spinach leaves

作　　者：徐静 王超 伍勇 吴秀丽 马泽刚 XU Jing;WANG Chao;WU Yong;WU Xiu-li;MA Ze-gang(Sichuan Vocational College of Health and Rehabilit,Zigong 643000,Sichuan,China)

机构地区：[1]四川卫生康复职业学院,四川自贡643000

出　　处：《湖北农业科学》2022年第7期83-88,94,共7页Hubei Agricultural Sciences

基　　金：自贡市重点科技计划项目(2020YGJC10);四川卫生康复职业学院校级项目(CWKY-2020Y-05);四川卫生康复职业学院生物资源开发与利用创新团队项目(CWKY-TD20-06)。

摘　　要：为进一步揭示Cs对植物光合作用的影响机理,采用不同浓度的CsCl和石英砂培处理菠菜(Spinacia oleracea L.)幼苗,利用快速叶绿素荧光诱导动力学分析手段,探究Cs胁迫后菠菜叶片光系统Ⅱ(PSII)的受损部位、受损程度及光合电子传递情况。结果表明,当CsCl处理浓度为1~5 mmol/L时,菠菜PSII反应中心供体侧放氧复合体(OEC)受到一定伤害,但不显著,与此同时,φ_(Po)、φ_(Eo)、Ψ_(o)参数值上升,说明低浓度处理下菠菜通过调节有活性的反应中心的开放程度升高,促使PSII光合活性增大,从而适应外界环境的变化;当CsCl处理浓度大于10 mmol/L时,CsCl胁迫既损伤了菠菜叶片PSII供体侧OEC,同时也对PSII的反应中心和受体侧造成伤害,从而阻碍光合电子的传递。In order to reveal the effect mechanism of Cs on plant photosynthesis,different concentrations of CsCl were used to treat spinach seedlings which were cultured in quartz sand. The chlorophyll fluorescence induction kinetic analysis method were used to explore damaged position,damaged degree and photosynthetic electron transfer of spinach leaf PSII after Cs stress. The results showed that the OEC on the donor side of the PSII reaction center of spinach suffered slight damage when the CsCl concentration is 1~5 mmol/L.In order to adapt to the change of external environment,the opening degree of active reaction centers in spinach were increased under low concentration treatment,which promotes the increase of PSII photosynthetic activity were reflected by the rised parameters of φ_(Po),φ_(Eo),Ψ_(o). Photosynthetic electron transport were blocked because of the damage on the PSII donor side OEC of spinach leaves,reaction center and receptor side of PSII when the concentrations of CsCl reach 10 mmol/L.

关 键 词：铯 菠菜(Spinacia oleracea L.) 光合机构 快速叶绿素荧光诱导动力学 JIP-测定 

分 类 号：S636.1[农业科学—蔬菜学] Q945[农业科学—园艺学]