阿尔泰冷蒿化学成分研究  

Research on the Chemical Constituents of Artemisia frigida Willd.in Altay

作　　者：李知瑾 郑丽华[1] 刘磊[1] LI Zhijin;ZHENG Lihua;LIU Lei(National Engineering Laboratory for Druggable Gene and Protein Screening,Northeast Normal University,Changchun 130024,China;Xiamen Health and Medical Big Data Center(Xiamen Medicine Research Institute)/Xiamen Key Laboratory of Natural Medicine Research and Development,Xiamen 361008,China)

机构地区：[1]东北师范大学药物基因与蛋白筛选国家工程实验室,吉林长春130024 [2]厦门市健康医疗大数据中心(厦门医药研究所)/厦门市天然药物研究与开发重点实验室,福建厦门361008

出　　处：《中国民族民间医药》2022年第8期38-44,共7页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：吉林省科技厅的资助项目(No.20190304026YY,20200201135JC,20200404124YY)。

摘　　要：目的:对阿尔泰冷蒿的化学成分进行研究。方法:利用液-液萃取、硅胶、葡聚糖凝胶、聚酰胺、大孔树脂、重结晶等手段,提取分离得到了10个单体化合物,其后通过理化常数测定、波谱数据分析及文献对照等手段鉴定了单体化合物的化学结构,利用MTT方法对单体化合物进行抗肿瘤活性的筛选。结果:从阿尔泰冷蒿中提取分离得到的化合物包括木栓酮(1)、豆甾-5-烯-3β,7α-二醇(2)、3,5-二羟基-6,7,3’,4’-四甲氧基黄酮(3)、蒿黄素(4)、苜蓿素(5)、猫眼草酚D(6)、Roxbughianin B(7)、木犀草素-7-O-β-D-葡萄糖苷(8)、苜蓿素-7-O-β-D-葡萄糖苷(9)、苜蓿素-4-O-β-D-葡萄糖苷(10),其中3,5-二羟基-6,7,3’,4’-四甲氧基黄酮(3)对人肝癌细胞系HepG2的IC_(50)值为(34.58±2.42)μg/mL。结论:从阿尔泰冷蒿中提取分离得到10个化合物。同时,首次对Roxbughianin B(7)的绝对构型进行解析。通过活性筛选发现,3,5-二羟基-6,7,3’,4’-四甲氧基黄酮(3)具有抗肿瘤活性。Objective To study the chemical constituents of Artemisia frigida Willd.in Altay.Methods Using liquid-liquid extraction,polyamide column chromatography,Sephadex LH-20 column chromatography,silica gel column chromatography,macroporous resin column chromatography and recrystallization,isolated from the 10 compounds.Results They are identified by the methods of the basis physicochemcial propertyes,spectral analysis and comparison with literature data.Compounds were elucidated as:friedelin(1);Stigmast-5-ene-3β,7α-diol(2);3,5-dihydroxy-6,7,3’,4’-trtramethoxy flavone(3);artemetin(4);tricin(5);chrysosplenol D(6);Roxbughianin B(7);luteolin-7-O-β-D-glucoside(8);tricin-7-O-β-D-gluco-pyranoside(9);tricin-4’-O-β-D-glucopyranside(10).The IC_(50) values of 3,5-dihydroxy-6,7,3’,4’-trtramethoxy flavone(3)was(34.58±2.42)μg/mL.Conclusion We extracted and identified 10 compounds.There were all firstly obtained from Artemisia frigida Willd.At the same time,we firstly identified absolute configuration of Roxbughianin B(7).3,5-dihydroxy-6,7,3’,4’-trtramethoxy flavone(3)have antitumor activity.

关 键 词：阿尔泰冷蒿 分离 鉴定 抗肿瘤 

分 类 号：R284.1[医药卫生—中药学]