基于PI3K/AKT/NF-κB通路探讨苗药血人参对D-半乳糖干预阿尔茨海默病模型小鼠大脑皮层的作用机制  

Based on PI3K/Akt pathway and NF-κB pathway,the mechanism of effect of miao medicine buerger lespedeza root on D-galactose intervention in cerebral cortex of Alzheimer’s disease model mice was investigated

作　　者：吴雅晨 唐冰雪 刘明 段丽 胡万福 刘刚 张永萍 Wu Yachen;Tang Bingxue;Liu Ming;Duan Li;Hu Wanfu;Liu Gang;Zhang Yongping(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学,贵州省贵阳市550025 [2]贵州中医药大学中药、民族药药理作用及作用机制研究中心 [3]贵州中医药大学第一附属医院

出　　处：《现代养生》2021年第22期10-13,共4页Health Protection and Promotion

基　　金：贵州省重大应用基础研究计划项目(编号:黔科合J重大字[2015]2002-3-5号);贵州省教育厅千层次人才项目(编号:贵中医[ZQ2015005]);贵州省科技计划项目(编号:黔科合基础[2019]1033号);全国中药特色技术传承人才培训项目(编号:国中医药人教函[2018]204号)。

摘　　要：目的研究苗药血人参对阿尔茨海默病(AD)模型小鼠大脑皮层磷酸肌醇3-激酶(PI3K)、蛋白激酶B(AKT)与核转录因子kappaB(NF-κB)信号通路的影响。方法选取SPF级KM小鼠60只,按照随机数表法随机分为6组,分别为空白对照组,模型组,血人参高、中、低剂量组,吡拉西坦组,每组10只。除空白对照组外,其余5组通过连续注射D-半乳糖(150mg/kg)、亚硝酸钠(50mg/kg)60d,建立AD小鼠模型,并在造模25d后开始灌胃给予相应药物治疗,空白对照组和模型组灌胃等容量生理盐水,血人参高、中、低剂量组,吡拉西坦组分别给予20g/kg、10g/kg、5g/kg的血人参提取液及0.8g/kg吡拉西坦片溶液。通过跳台实验检测各组小鼠学习记忆能力,免疫组化法检测各组小鼠大脑皮层PI3K、AKT、NF-κB、肿瘤坏死因子-α(TNF-α)蛋白的表达情况。结果与空白对照组相比,模型组的学习记忆能力明显下降,差异有统计学意义(P<0.01),PI3K、AKT、NF-κB、TNF-α、的表达显著上调,差异有统计学意义(P<0.05,P<0.01)。与模型组相比,各治疗组小鼠的学习记忆能力明显提高,差异有统计学意义(P<0.05,P<0.01),PI3K、AKT、NF-κB、TNF-α的表达显著下调,差异有统计学意义(P<0.05,P<0.01)。结论血人参对AD模型小鼠学习记忆障碍有改善作用,其作用机制可能与调控PI3K/AKT/NF-κB信号通路,抑制炎症因子TNF-α的表达有关。Objective To study the effects of Miao medicine buerger lespedeza root on the inositol phosphate 3-kinase(PI3 K)/protein kinase B(AKT)and nuclear transcription factor kappaB(NF-κB)signaling pathway in cerebral cortex of Alzheimer’s disease(AD)model mice.Methods A total of 60 SPF KM mice were randomly divided into blank control group,model group,buerger lespedeza root high,medium and low dose group,and piracetam group with 10 mice in each group.In addition to the blank control group,each group the D-galactose mice injected with sodium nitrite for 60 days,AD mice model was established,and the building 25 days began to fill the stomach to give corresponding drug therapy,such as blank control group and model group to fill the stomach capacity of physiological saline,the treatment group were given different concentrations of buerger lespedeza root extract and pyrazole raschig chip solution.The learning and memory abilities of mice in each group were tested by jumping off experiment,and the protein expressions of NF-κB,tumor necrosis factor-α(TNF-α),PI3 K,and AKT in the cerebral cortex of mice in each group were detected by immunohistochemistry.Results Compared with the blank control group,the learning and memory ability of the model group was significantly decreased(P<0.01),and the expressions of NF-κB,TNF-α,PI3 K,and AKT were significantly up-regulated(P<0.05,P<0.01).Compared with the model group,the learning and memory ability of mice in each treatment group was significantly increased(P<0.05,P<0.01),and the expressions of NF-κB,TNF-α,PI3 K,and AKT were significantly down-regulated(P<0.05,P<0.01).Conclusion Buerger lespedeza root can improve learning and memory impairment in AD model mice.Its mechanism may be related to the regulation of NF-κB signaling pathway and inhibiting the expression of NF-κB,TNF-α,PI3 K,AKT.

关 键 词：苗药 血人参 阿尔茨海默病 学习记忆能力 核转录因子KAPPAB 

分 类 号：Q95-33[生物学—动物学]