针刺干预对单眼视觉剥夺模型大鼠视皮层方位柱“漂移”和异常重组的调节作用及其脑功能机制  

Regulatory effect of acupuncture intervention on“drift”and abnormal reorganization of visual cortical orientation columns in rat model of monocular visual deprivation and its mechanism of brain function

作　　者：叶钰娟 张娟娟 马重兵 严兴科 YE Yujuan;ZHANG Juanjuan;MA Chongbing;YAN Xingke(Acumox and Tuina College,Gansu University of Traditional ChineseMedicine,lanzhou 730000,China)

机构地区：[1]甘肃中医药大学针灸推拿学院,甘肃兰州730000

出　　处：《吉林大学学报（医学版）》2022年第2期291-298,共8页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金项目(81860879,82160935)。

摘　　要：目的:探讨针刺干预对大鼠单眼视觉剥夺后视皮层方位柱“漂移”和通道重组的影响,初步阐明其作用机制。方法:将70只健康SD大鼠随机分为空白对照组10只,剩余60只先采用右眼缝合复制视觉剥夺模型,筛选出模型复制成功的50只大鼠,再随机分为模型组,左旋多巴甲酯(LDME)组,早期(模型复制后第3天)、中期(模型复制后第12天)和末期(模型复制后第21天)针刺组,每组10只,给予相应干预后,采用功能近红外光谱成像(f NIRs)技术检测各组大鼠不同方位刺激状态下视皮层方位柱含氧血红蛋白(Oxy-Hb)、脱氧血红蛋白(Deoxy-Hb)和总血红蛋白(Total-Hb)浓度。结果:与空白对照组比较,模型组大鼠2-5和4-4通道双侧视皮层中Oxy-Hb浓度均明显降低(P<0.05);与模型组比较,早、中和晚期针刺组大鼠2-5和4-4通道Oxy-Hb及Total-Hb浓度均明显升高(P<0.05);2-5和4-4通道双侧视皮层Deoxy-Hb浓度明显降低(P<0.05);空白对照组大鼠2-5和4-4通道左侧及右侧上述3种血红蛋白浓度比较差异无统计学意义(P>0.05),模型组大鼠上述3种血红蛋白浓度右侧均高于左侧(P<0.01);LDME组大鼠Oxy-Hb和Deoxy-Hb浓度右侧高于左侧(P<0.05),Total-Hb浓度比较差异无统计学意义(P>0.05);早期针刺组大鼠Deoxy-Hb和Total-Hb浓度右侧高于左侧(P<0.05),Oxy-Hb浓度比较差异无统计学意义(P>0.05);中期和末期针刺组大鼠视皮层上述3种血红蛋白右侧均高于左侧(P<0.05)。结论:单(右)眼视觉剥夺后,对侧视皮质神经元方位信息感知的敏感性和选择性明显抑制,同侧视皮层方位柱功能异常重组;早期针刺能有效调节视觉剥夺后视皮层方位柱“漂移”和异常重组。Objective:To explore the effect of acupuncture intervention on the“drift”and channel reorganization of visual cortex azimuth column after monocular visual deprivation of the rats,and to preliminarily clarify mechanism. Methods:A total of 70 healthy SD rats were randomly divided into blank control group(n=10)and right eye suture group(n=60). The rats in right eye suture group were used to establish the visual deprivation models by right eye suture. Then the successful model rats were randomly divided into model group,levodopa methyl ester(LDME)group and early(3 d after model replication),middle(12 d after model replication)and late(21 d after model replication)acupuncture groups(n=10).After the corresponding intervention,the concentrations of oxyhemoglobin(Oxy-Hb),deoxygenated hemoglobin(Deoxy-Hb)and total hemoglobin(Total-Hb)of the rats in various groups were detected by functional near infrared spectroscope(f NIRs)technique. Results:Compared with blank control group,the concentrations of Oxy-Hb in bilateral visual cortex of 2-5 and 4-4 channels of the rats in model group were significantly decreased(P<0. 05);compared with model group,the concentrations of Oxy-Hb and Total-Hb in 2-5 and 4-4 channels in early,middle and late acupuncture groups were significantly increased(P<0. 05);the concentrations of Deoxy-Hb were decreased;there were no significant differences in the concentrations of Oxy-Hb,Deoxy-Hb and Total-Hb between the left and right sides of 2-5 and 4-4 channels in blank control group(P>0. 05),but the concentration of Oxy-Hb in the right side of the rats in model group was higher than that in the left side(P<0. 01). In LDME group,the concentrations of Oxy-Hb and Deoxy-Hb in the right side was higher than that in the left side(P<0. 05),but there was no difference in the concentration of Total-Hb(P>0. 05). In early acupuncture group,the Oxy-Hb concentration of rats had no difference between the left and right sides(P>0. 05),while the concentrations of Deoxy-Hb and Total-Hb in the right side were higher

关 键 词：弱视 针刺 单眼剥夺 视皮层方位柱 功能近红外光谱技术 脑功能 

分 类 号：R246.9[医药卫生—针灸推拿学]