枇杷果肉颜色调控关键基因PSY2A的分子标记开发与应用  

Development and Application of Molecular Marker for the Key Gene EjPSY2A of Flesh Color Regulation in Loquat

作　　者：宋海岩[1,2] 孙淑霞[2] 陈栋[2] 李靖[2] 涂美艳[2] 徐子鸿 娄红霞 龚荣高[1] 江国良 SONG Haiyan;SUN Shuxia;CHEN Dong;LI Jing;TU Meiyan;XU Zihong;LOU Hongxia;GONG Ronggao;JIANG Guoliang(College of Horticulture,Sichuan Agricultural University,Chengdu 611130,China;Horticulture Research Institute of Sichuan Academy of Agricultural Sciences,Southwestern Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement,Ministry of Agriculture and Rural Affairs,Chengdu 610066,China)

机构地区：[1]四川农业大学园艺学院,成都611130 [2]四川省农业科学院园艺研究所&农业农村部西南地区园艺作物生物学与种质创制重点实验室,成都610066

出　　处：《四川农业大学学报》2022年第2期172-178,205,共8页Journal of Sichuan Agricultural University

基　　金：第三次全国农作物种质资源普查与收集行动(19210125);四川省农作物畜禽育种攻关(21ZDYF2196);四川省主要经济作物分子育种平台(2021YFYZ0010);特色水果四川省青年科技创新研究团队(20CXTD0041);国家成都农业科技中心地方财政专项资金项目(NASC2021AT04)。

摘　　要：【目的】为开发准确的枇杷果肉颜色早龄鉴定分子标记,加速枇杷品种改良进程。【方法】基于枇杷八氢番茄红素合成酶基因EjPSY2A在黄肉和白肉枇杷品种间的序列差异设计2对InDel分子标记,经初步筛选后选取分型结果清晰的引物PSY2A-2对85份枇杷种质资源与杂交后代分型鉴定的准确性进行验证,并通过TA克隆方法对EjPSY2A位点扩增片段的正确性进一步验证。【结果】所有42份白肉枇杷材料具有纯合的EjPSY2A^(d)基因型,而43份黄肉枇杷材料全部为纯合的EjPSY2A基因型或者EjPSY2A^(d)与EjPSY2A杂合基因型,并通过TA克隆的方法证实了‘大五星’枇杷的扩增大片段为纯合EjPSY2A等位基因。【结论】开发的PSY2A-2分子标记可以准确地将黄肉和白肉枇杷种质资源区分,对指导枇杷杂交后代早龄选择育种具有重要意义,有助于加速枇杷育种工作的进程。【Objective】This paper aims to develop a reliable molecular marker for loquat flesh color identification at juvenile period so as to accelerate the cultivar improvement process.【Method】In this study,we designed two InDel molecular markers were designed based on the sequence difference of phytoene synthase gene EjPSY2A between yellow-and white-fleshed loquat varieties. A primer PSY2A-2 with clear genotyping results was selected after preliminary screening,and then its identification accuracy was detected with 85 loquat germplasm resources and hybrids worldwide,also the sequence of amplification product with the primer PSY2A-2 was further confirmed using TA cloning method.【Result】The results showed that all 42 white-fleshed loquat samples possessed homozygous EjPSY2A^(d) genotype,all 43 yellow-fleshed loquat samples possessed homozygous EjPSY2A genotype or heterozygous EjPSY2A and EjPSY2A^(d) genotype. It was confirmed that the amplification product of ‘Dawuxing’was a homozygous EjPSY2A allele with TA cloning method.【Conclusion】The newly-developed InDel molecular marker PSY2A-2 can accurately distinguish the yellow-and white-fleshed loquat germplasm resources,which has a significant meaning for early selective breeding in loquat hybrids and can help to accelerate the process of loquat breeding.

关 键 词：枇杷 果肉颜色 分子标记 EjPSY2A 

分 类 号：S661.9[农业科学—果树学]