Chitin deacetylase:from molecular structure to practical applications  

作　　者：Ziyang Huang Xueqin Lv Guoyun Sun Xinzhu Mao Wei Lu Yanfeng Liu Jianghua Li Guocheng Du Long Liu 

机构地区：[1]Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China [2]Science Center for Future Foods,Jiangnan University,Wuxi 214122,China [3]Shandong Runde Biotechnology Co.,Ltd.,Tai’an 271000,China

出　　处：《Systems Microbiology and Biomanufacturing》2022年第2期271-284,共14页系统微生物学与生物制造（英文）

基　　金：This work was financially supported by the Key Research and Development Program of China(2018YFA0900300);the National Natural Science Foundation of China(32021005,31870069);the Fundamental Research Funds for the Central Universities(JUSRP52019A,JUSRP121010,JUSRP221013);Shandong Province Key R&D Program(Major Science and Technology Innovation Project)Project(2019JZZY011002).

摘　　要：Chitosan and chitooligosaccharides(COS),as derivatives of chitin through deacetylation reaction,have broad applications due to their good biodegradability,biocompatibility,and solubility.In addition,chitosan and COS are involved in cell wall morphogenesis and host–pathogen interactions in vivo.Chitin deacetylases(CDAs)are enzymes that can catalyze the deN-acetylation of chitin.They are widely distributed in protozoa,algae,bacteria,fungi,and insects with important physiological functions.Compared with the traditional chemical method,enzymatic catalysis by CDAs provides an enzymatic catalysis method to produce chitosan and COS with controllable deacetylation site and environmental friendliness.These characteristics attract researchers to produce CDAs by fungicides or pesticides.However,researches on heterologous expression and directed evolution of CDAs are still lacking.In this review,we summarize the latest knowledge of CDAs,especially for heterologous expression systems and directed evolution strategies,which may contribute to the industrial production and future application of CDAs.

关 键 词：Chitin deacetylases CHITOSAN CHITOOLIGOSACCHARIDES Heterologous expression Directed evolution 

分 类 号：O64[理学—物理化学]