温通活血乳膏皮肤给药可减轻糖尿病周围神经病变模型大鼠的炎症反应  

Skin administration of Wentong Huoxue Cream can relieve inflammation in diabetic peripheral neuropathy rats

作　　者：叶婷 李晶[1] 徐利娟 马丽[1] Ye Ting;Li Jing;Xu Lijuan;Ma Li(Department of Endocrinology,the Fourth Affiliated Hospital of Xinjiang Medical University,Urumqi 830061,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]新疆医科大学第四附属医院内分泌科,新疆维吾尔自治区乌鲁木齐市830061

出　　处：《中国组织工程研究》2022年第32期5091-5096,共6页Chinese Journal of Tissue Engineering Research

基　　金：新疆维吾尔自治区自然科学基金项目(2019D01C174),项目负责人:叶婷。

摘　　要：背景:温通活血乳膏具有改善氧化应激、降低血黏度、恢复微循环、减轻糖尿病周围神经损伤、抑制感觉传导和痛觉形成的作用,但其调控作用机制尚不清楚。目的:观察温通活血乳膏对糖尿病周围神经病变大鼠JNK信号通路蛋白及炎性因子的影响。方法:取成年雄性Wistar大鼠,建立糖尿病周围神经病变模型。将造模成功的40只大鼠随机分5组,每组8只,模型组、西药组后肢涂抹空白乳膏2 mL,其中西药组每天灌胃给予弥可保0.14 mg/kg,低、中、高剂量组后肢分别涂抹0.75,1.5,3.0 g/mL的温通活血乳膏2 mL,药膏涂抹2次/d,连续给药28 d;同时设立正常组(不造模不给药,n=6)。末次给药后,进行神经传导检测,采用ELISA法检测血清中白细胞介素6、白细胞介素18、肿瘤坏死因子α、超敏C-反应蛋白、髓鞘碱性蛋白的水平,Western blot法检测坐骨神经节中JNK、p-JNK的蛋白表达,RT-PCR法检测JNK mRNA的表达。结果与结论:①与正常组比较,模型组大鼠感觉与运动神经传导速度降低(P <0.05)。与模型组比较,中、高剂量组和西药组大鼠感觉神经传导速度升高(P <0.05),其中以高剂量组升高最明显(P <0.05);高剂量组和西药组大鼠运动神经传导速度升高(P <0.05)。②与正常组比较,模型组白细胞介素6、肿瘤坏死因子α、高敏C-反应蛋白、髓鞘碱性蛋白水平升高(P <0.05),白细胞介素18水平降低(P <0.05)。与模型组比较,中、高剂量组与西药组白细胞介素6、白细胞介素18、肿瘤坏死因子α、高敏C-反应蛋白、髓鞘碱性蛋白水平降低(P <0.05),其中以高剂量组、西药组效果更明显。③与正常组比较,模型组p-JNK蛋白及JNK mRNA表达水平升高(P <0.05)。与模型组比较,中、高剂量组及西药组p-JNK蛋白及JNK mRNA表达水平降低(P <0.05)。④结果表明,温通活血乳膏能够抑制JNK信号转导通路的异常激活,减轻糖尿病周围神经病变大鼠的炎症反应BACKGROUND:Wentong Huoxue Cream can improve oxidative stress,reduce blood viscosity,resto re mic rocirculation,relieve diabetic peripheral nerve injury,and inhibit sensory conduction and pain formation,but its regulato ry mechanism remains unclear.OBJECTIVE:To observe the effects of Wentong Huoxue Cream on JNK signaling pathway proteins and inflammatory factors in diabetic peripheral neuropathy rats.METHODS:A rat model of diabetic peripheral neuropathy was established in adult Wistar rats.Forty diabetic peripheral neuro pathy rats were randomly divided into five groups(n=8 per group):model group,low-,middle-and high-dose Wentong Huoxue Cream groups(given 2 mL of 0.75,1.5,3.0 g/mL Wentong Huoxue Cream to the lower limbs of rats,respectively) and Western medicine group(given 2 mL of control cream).Skin treatment in each group was performed twice a day for 28 days.Additionally,six rats were taken as normal group.After the last administration,nerve conduction tests were performed and the expressions of inte rleukin-6,interleukin-18,tumor necrosis factor a,high-sensitivity C-reactive protein,and myelin basic protein in serum were detected by ELISA.The expression of JNK and P-JNK protein in sciatic ganglia was detected by western blot and the expression of JNK mRNA was determined by RT-PCR.RESULTS AND CONCLUSION:The velocity of sensory nerve conduction and motor nerve conduction was significantly decreased in the model group compared with the normal group(P <0.05).Compared with the model group,the sensory nerve conduction velocity increased significantly after intervention with different doses of Wentong Huoxue Cream(P<0.05),especially in the high-dose Wentong Huoxue Cream group(P<0.05).Compared with the model group,the motor nerve conduction velocity was significantly increased in the high-dose Wentong Huoxue Cream group and Western medicine group(P <0.05).Compared with the normal group,the serum levels of interleukin-6,tumor necrosis factor α,high-sensitivity C-reactive protein,and myelin basic protein were signific

关 键 词：温通活血乳膏 糖尿病 周围神经病变 大鼠 JNK信号通路 炎性因子 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]