非编码RNA调节心肌缺血再灌注损伤中自噬的作用及机制  

Effect and mechanism of non-coding RNA regulating autophagy in myocardial ischemia-reperfusion injury

作　　者：范吉林 朱婷婷 田晓玲[2] 刘思佳 苏静[3] 张世亮[3] Fan Jilin;Zhu Tingting;Tian Xiaoling;Liu Sijia;Su Jing;Zhang Shiliang(Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong Province,China;Affiliated Hospital of Binzhou Medical College,Binzhou 256600,Shandong Province,China;Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong Province,China)

机构地区：[1]山东中医药大学,山东省济南市250014 [2]滨州医学院附属医院,山东省滨州市256600 [3]山东中医药大学附属医院,山东省济南市250014

出　　处：《中国组织工程研究》2022年第35期5716-5723,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家科技重大专项重大新药创制项目(2017ZX09301003);项目负责人:张世亮。

摘　　要：背景:越来越多的研究表明自噬在心肌缺血再灌注损伤中发挥重要作用,适度的自噬有助于维持心脏的正常和代谢功能。非编码RNAs包括长链非编码RNA(lncRNAs)、环状RNA(circRNAs)和微小RNA(miRNAs),能够通过调控自噬参与维持心肌缺血再灌注损伤进程,从而维持心肌细胞稳态和保护心肌细胞。目的:对非编码RNA在心肌缺血再灌注损伤中调节自噬的作用和分子机制进行了综述,以期为心肌缺血再灌注损伤甚至其他相关心血管疾病的治疗奠定基础。方法:以“noncoding RNAs,non-coding RNAs,long non-coding RNA,circ RNA,micro RNA,mi RNA,autophagy,myocardial ischemia-reperfusion injury”“非编码RNA,自噬,心肌缺血再灌注损伤”为关键词在PubMed、Web of science、万方和中国知网数据库检索2011年1月至2021年11月的相关文献。制定纳入和排除标准,通过阅读文献标题、摘要及全文内容进行筛选,最终纳入78篇相关文献。结果与结论:(1)自噬在心肌缺血再灌注损伤的病理学中起着双重作用。在生理条件下,适度的自噬通过降解和回收受损的细胞器和蛋白质来维持细胞代谢平衡,促进心肌细胞存活,但过度自噬会加重心肌细胞损伤。(2)非编码RNA调控自噬可能成为诊断和药物开发的新靶点。自噬与非编码RNA的机制研究可以为心肌缺血再灌注损伤的防治提供新的策略。BACKGROUND:Increasing studies have shown that autophagy plays an important role in myocardial ischemia-repe rfusion injury and moderate autophagy help maintain the normal and metabolic function of the heart.Non-coding RNAs,including long non-coding RNAs,circular RNAs,and microRNAs,can maintain cardiomyocyte homeostasis and protect cardiomyocytes by regulating autophagy.OBJECTIVE:To review the role and molecular mechanism of non-coding RNAs in regulating autophagy in myocardial ischemia-reperfusion injury,in order to lay a foundation for the treatment of myocardial ischemia-repe rfusion injury and other related cardiovascular diseases.METHODS:PubMed,Web of Science,Wan Fang and CNKI databases were searched for relevant literature published from January 2011 to January 2021 using the keywords of"noncoding RNAs,non-coding RNAs,long non-coding RNA,circRNA,microRNA,miRNA,autophagy,myocardial ischemia-repe rfusion injury"in English and Chinese.The inclusion and exclusion criteria were formulated and 78 related documents were finally included by reading the titles,abstracts and full-text contents.RESULTS AND CONCLUSION:Autophagy plays a dual role in the pathology of myocardial ischemia-repe rfusion injury.Under physiological conditions,moderate autophagy can maintain the balance of cell metabolism and promote the survival of cardiomyocytes by degrading and recovering damaged organelles and proteins,but excessive autophagy will aggravate the damage of cardiomyocytes.Non-coding RNA regulating autophagy may become a new target for the development of diagnosis and therapeutic drugs.In addition,it is reasonable to think that the study on the mechanism of autophagy and non-coding RNAs can provide a new strategy for the prevention and treatment of myocardial ischemia-reperfusion injury.

关 键 词：非编码RNA lncRNA circRNA MIRNA 心肌缺血再灌注损伤 自噬 综述 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]