SCAMP2与Rab8a在小鼠原代巨噬细胞源性泡沫细胞胆固醇转运中的作用  

Role of SCAMP2 and Rab8a in cholesterol transport of primary mouse macrophage-derived foam cells

作　　者：林俍良 潘海强 丁航 马卫列 张志珍 LINLiang-liang;PAN Hai-qiang;DING Hang;MA Wei-lie;ZHANG Zhi-zhen(Department of Biochemistry and Molecular Biology,Guangdong Medical University,Dongguan 523808,China)

机构地区：[1]广东医科大学生物化学与分子生物学教研室,广东东莞523808

出　　处：《广东医科大学学报》2022年第2期132-136,共5页Journal of Guangdong Medical University

基　　金：广东省基础与应用基础研究基金自然科学基金(2020A1515010224)。

摘　　要：目的探讨分泌载体膜蛋白2(SCAMP2)与GTP酶Rab8a在小鼠原代腹腔巨噬细胞源性泡沫细胞胆固醇转运中的相互作用。方法8周龄雄性C57BL/6NJ小鼠提取腹腔巨噬细胞,加入50 mg/L乙酰化低密度脂蛋白(acLDL)诱导48 h,建立巨噬细胞源性泡沫细胞模型;加入10 mg/L载脂蛋白A-1(apoA-1)12 h诱导泡沫细胞内胆固醇流出,用qRT-PCR、Western blot、免疫荧光检测SCAMP2、Rab8a表达,免疫共沉淀观察SCAMP2、Rab8a蛋白相互作用。结果泡沫细胞内SCAMP2和Rab8a表达增加,且存在相互作用;apoA-1处理上调泡沫细胞中SCAMP2和Rab8a表达、相互作用及共定位。结论SCAMP2与Rab8a可能在apoA-1诱导泡沫细胞胆固醇外流过程中起协同促进作用。Objective To investigate the interactionbetween secretory carrier membrane protein 2(SCAMP2)and GTPase Rab8a in cholesterol transport of primary mouse peritoneal macrophage-derived foam cells.Methods The peritoneal macrophages were isolated from 8-week-old male C57BL/6NJ mice and incubated with 50 mg/L acetylated lowdensity lipoprotein(acLDL)for 48 h to induce foam cells.The cholesterol efflux of foam cells was induced by treatment with 10 mg/L apolipoprotein A-1(apo A-1)for 12 h.Expression of SCAMP2 and Rab8a and their interaction were determined by qRT-PCR,Western blot,immunofluorescence and co-immunoprecipitation.Results Overexpression and interaction of SCAMP2 and Rab8a were found,and apoA-1 treatment upregulated their expression,interaction,and colocalization in foam cells.Conclusion Both SCAMP2 and Rab8a could play a synergistic role in promoting cholesterol efflux from foam cells induced by apoA-1.

关 键 词：巨噬细胞 泡沫细胞 SCAMP2 Rab8a 载脂蛋白A-1 

分 类 号：R54[医药卫生—心血管疾病]