A 3D-printable device allowing fast and reproducible longitudinal preparation of mouse intestines  

作　　者：Beckey DeLucia Sergey Samorezov Megan T.Zangara Rachel L.Markley Lucas J.Osborn Karlee B.Schultz Christine McDonald Jan Claesen 

机构地区：[1]Department of Cardiovascular and Metabolic Sciences,Lerner Research Institute,Cleveland Clinic,Cleveland,Ohio,USA [2]Center for Microbiome and Human Health,Lerner Research Institute,Cleveland Clinic,Cleveland,Ohio,USA [3]Department of Biomedical Engineering,Lerner Research Institute,Cleveland Clinic,Cleveland,Ohio,USA [4]Department of Molecular Medicine,Cleveland Clinic Lerner College of Medicine of Case Western Reserve University,Cleveland,Ohio,USA [5]Department of Inflammation and Immunity,Lerner Research Institute,Cleveland Clinic,Cleveland,Ohio,USA [6]College of Arts and Sciences,John Carroll University,University Heights,Ohio,USA

出　　处：《Animal Models and Experimental Medicine》2022年第2期189-196,共8页动物模型与实验医学（英文）

基　　金：This study was supported by a research grant from the Prevent Cancer Foundation(PCF2019;J.C.);seed funding from the Cleveland Clinic Foundation(J.C.);a National Institutes of Health grant(R01 AI153173;J.C.);an American Cancer Society Institutional Research Grant(IRG-16-186-21;J.C.);a Jump Start Award from the Case Comprehensive Cancer Center(CA043703;J.C.);funding from the Office of the Assistant Secretary of Defense for Health Affairs through the Congressionally Directed Medical Research Programs Peer Reviewed Medical Research Program under award no.W81XWH-19-1-0488(PR181846;C.M.)。

摘　　要：Accurate and reproducible analysis of murine small and large intestinal tissue is key for preclinical models involving intestinal pathology.Currently,there is no easily ac-cessible,standardized method that allows researchers of different skill levels to con-sistently dissect intestines in a time-efficient manner.Here,we describe the design and use of the 3D-printed“Mouse Intestinal Slicing Tool”(MIST),which can be used to longitudinally dissect murine intestines for further analysis.We benchmarked the MIST against a commonly used procedure involving scissors to make a longitudinal cut along the intestines.Use of the MIST halved the time per mouse to prepare the intestines and outperformed alternative methods in smoothness of the cutting edge and overall reproducibility.By sharing the plans for printing the MIST,we hope to contribute a uniformly applicable method for saving time and increasing consistency in studies of the mouse gastrointestinal tract.

关 键 词：ADENOMA DISSECTION i ntestines mice PRINTING three-dimensional reproducibility of results 

分 类 号：R574[医药卫生—消化系统]