Structural analysis of processed corneas  

作　　者：Shaoyang Anthony Yeh Hua Ye Zhanfeng Cui 

机构地区：[1]Institute of Biomedical Engineering,University of Oxford,Oxford,UK

出　　处：《Annals of Eye Science》2018年第1期76-81,共6页眼科学年鉴（英文）

摘　　要：Background:Disruption of the microstructure in corneal stroma can lead to the loss of transparency.The lack of a characterization method for the microstructure prevents such scaffolds to be implemented in tissue transplantation.The non-invasive,three-dimensional(3D)rendering multiphoton microscopy(MPM)poses the potential to solve this problem.Methods:MPM images and data analyses were performed with three kinds of samples with known and different quality.Isosurfaces(ISOs)were constructed for the evaluation of void volume and collagen distribution.Results:The differences in the microstructures of these samples were revealed with clear indications and links to their behaviours in rehydration and possible transparency.According to this analysis,the scaffold with the highest void space ratio amongst the three presented the highest successful rates to be thoroughly rehydrated.Conclusions:Such a method can be developed for assessing the quality of tissue engineered corneas,or donated corneas,and be useful as a powerful research tool in cornea related research.

关 键 词：Corneal transplantation tissue engineering multiphoton microscopy(MPM) second harmonic generation(SHG) isosurface(ISO) 

分 类 号：R31[医药卫生—基础医学]