Annals of Eye Science, 2018 7 © Annals of Eye Science. All rights reserved. Ann Eye Sci 2018;3:AB007 aes.amegroups.com  

作　　者：Stéphanie Proulx 

机构地区：[1]Centre de Recherche du CHU de Québec-UniversitéLaval,Axe Médecine Régénératrice,Hôpital du Saint-Sacrement,Québec,Canada [2]Centre de Recherche en Organogénèse Expérimentale de l'UniversitéLaval/LOEX,Québec,Canada [3]Département d'Ophtalmologie et d'Oto-Rhino-Laryngologie-Chirurgie Cervico-Faciale,Facultéde Médecine,UniversitéLaval,Québec,Canada

出　　处：《Annals of Eye Science》2018年第1期413-413,共1页眼科学年鉴（英文）

摘　　要：Background:The goal of this study was to engineer an epithelialized and endothelialized pigmented choroidal substitute using the self-assembly approach of tissue engineering.Methods:Cells from human choroids were isolated and cultured.Culture purity was assessed using immunostaining(CD31,HMB45,vimentin,keratins 8/18).To engineer the choroid,fibroblasts were cultured in the presence of serum and ascorbic acid to promote extracellular matrix(ECM)assembly.Endothelial cells,melanocytes or RPE cells were separately seeded on the stromal substitutes.Choroidal substitutes were further characterized by histology,mass spectrometry,immunostaining,and compared to native human choroids.Results:The technique used to isolate choroidal cells yielded pure cultures of fibroblasts,melanocytes and vascular endothelial cells.The stromal substitutes engineered using the self-assembly approach were composed of collagen(types I,VI,XII and XIV),proteoglycans(decorin,lumican)and other ECM proteins.Protein expression was confirmed using immunostaining.Endothelial cells spontaneously assembled into capillary-like structures and vascular networks when cocultured with fibroblast-containing ECM sheets.Conclusions:This study shows that the self-assembly approach of tissue engineering can be used to reconstruct a choroid using native cells.This model represents a unique tool to better understand the crosstalk between the different choroidal cell types and cell-ECM interactions.

关 键 词：Tissue engineering CHOROID SELF-ASSEMBLY 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学]