泡桐WPR基因家族鉴定及其对丛枝植原体的响应  

Identification of the WPR gene family in Paulownia and its response to witches′ broom phytoplasma

作　　者：常梦悦 曹喜兵[1] 赵振利[1] 范国强[1] CHANG Mengyue;CAO Xibing;ZHAO Zhenli;FAN Guoqiang(Institute of Paulownia,Henan Agricultural University,Zhengzhou,Henan 450002,China)

机构地区：[1]河南农业大学泡桐研究所,河南郑州450002

出　　处：《森林与环境学报》2022年第3期312-319,共8页Journal of Forest and Environment

基　　金：中原学者科学家工作室建设专项(豫科[2018]185)。

摘　　要：为研究WPR基因家族在泡桐丛枝病叶片黄化中的作用,在泡桐全基因组水平上,采用生物信息学方法对WPR基因家族成员进行鉴定、进化分析、染色体定位、共线性、基因结构、蛋白性质、顺式作用元件和表达量的研究。结果表明,白花泡桐基因组中共存在16个WPR基因,分布在9条染色体上,其中,12个基因具有种内共线性,9个基因与拟南芥具有共线性;所有WPR家族成员均含光响应、防御响应及激素响应元件。通过对植原体侵染后的转录组和互作蛋白分析,发现PfWEB3、PfWPRb2、PfWPRb3和PfPMI2可能与丛枝植原体侵染后泡桐叶片黄化有关。To study the role of the WPR gene family in leaf yellowing owing to Paulownia witches′ broom, bioinformatics methods were used to identify the WPR gene family members and their evolutionary relationship, chromosome localization, synteny, gene structure, protein nature, and cis-acting elements, and transcriptional expression at the genome-wide level. The results indicates 16 WPR genes in the P. fortunei genome that were distributed on 9 chromosomes, among which 12 genes had intraspecific collinearity in P. fortunei species, while 9 genes had collinearity with Arabidopsis thaliana. All WPR family members contained light, stress, and hormone response elements. Transcriptome data analysis of witches′ broom phytoplasma and the reciprocal protein revealed that PfWEB3, PfWPRb2, PfWPRb3, and PfPMI2 might be involved in the yellowing of Paulownia witches′ broom.

关 键 词：白花泡桐 WPR基因 家族进化 植原体 全基因组 染色体定位 生物信息学 

分 类 号：S763[农业科学—森林保护学]