五味子配伍对补骨脂致肝细胞氧化损伤和内质网应激的影响  

Effects of compatibility of Schisandra chinensis on Psoralea corylifolia-induced oxidative damage and endoplasmic reticulum stress in hepatocytes

作　　者：张婧茜 殷佳[2] 曲晓琳 刘慧 王娜 闫佳靓 傅春升[3] ZHANG Jingxi;YIN Jia;QU Xiaolin;LIU Hui;WANG Na;YAN Jiajing;FU Chunsheng(School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Dept.of Breast and Thyroid Surgery,the Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,China;Dept.of Science and Technology,the Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,China)

机构地区：[1]山东中医药大学药学院,济南250355 [2]山东中医药大学附属医院乳腺甲状腺外科,济南250014 [3]山东中医药大学附属医院科技处,济南250014

出　　处：《中国药房》2022年第9期1088-1093,共6页China Pharmacy

基　　金：山东省中医药科技发展计划项目(No.2019-0079)。

摘　　要：目的研究五味子配伍对补骨脂致L02肝细胞氧化损伤和内质网应激的影响,为补骨脂-五味子配伍减毒的临床应用提供参考。方法将L02细胞分为空白对照组、补骨脂模型组(1200μg/mL补骨脂,以生药量计)和补骨脂-五味子低、中、高剂量组(1200μg/mL补骨脂分别联用600、1200、2400μg/mL五味子,以生药量计)。各组细胞加培养液或药液培养48 h后,检测细胞中天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)水平,检测细胞培养液中谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平,检测细胞中活性氧(ROS)水平及细胞线粒体膜电位,检测细胞中葡萄糖调节蛋白78(GRP-78)、蛋白激酶R样内质网激酶(PERK)mRNA及其蛋白表达水平。结果与空白对照组比较,补骨脂模型组细胞(或其培养液)中AST、ALT、MDA、ROS水平和GRP-78、PERK mRNA及其蛋白表达水平均显著升高(P<0.05或P<0.01),GSH、SOD水平和线粒体膜电位均显著降低(P<0.05或P<0.01);与补骨脂模型组比较,补骨脂-五味子低、中、高剂量组上述指标均显著改善(P<0.05或P<0.01)。结论五味子与补骨脂配伍后可以缓解补骨脂引起的L02细胞氧化损伤和内质网应激。OBJECTIVE To study the effects of the compatibility of Schisandra chinensis on Psoralea corylifolia-induced oxidative damage and endoplasmic reticulum stress in L02 hepatocytes,and to provide reference for clinical use of the compatibility attenuation of P.corylifolia-S.chinensis.METHODS L02 cells were divided into blank control group,P.corylifolia model group(1200μg/mL P.corylifolia,calculated by crude drug),P.corylifolia-S.chinensis low-dose,medium-dose and high-dose groups(1200μg/mL P.corylifolia combined with 600,1200,2400μg/mL S.chinensis,respectively,calculated by crude drug).After the cells in each group were cultured in culture medium or drug solution for 48 hours,the levels of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)were detected;the levels of glutathione(GSH),superoxide dismutase(SOD)and malondialdehyde(MDA)in cell culture medium were detected;reactive oxygen species(ROS)level and mitochondrial membrane potential in cells were detected;mRNA and protein expressions of glucose-regulated protein 78(GRP-78)and protein kinase R-like endoplasmic reticulum kinase(PERK)were detected.RESULTS Compared with blank control group,the levels of AST,ALT,MDA and ROS,mRNA and protein expressions of GRP-78 and PERK were increased significantly in P.corylifolia model group(P<0.05 or P<0.01);while GSH and SOD levels,mitochondrial membrane potential were decreased significantly(P<0.05 or P<0.01).Compared with P.corylifolia model group,above indexes of P.corylifolia-S.chinensis low-dose,medium-dose and high-dose groups were all improved significantly(P<0.05 or P<0.01).CONCLUSIONS The compatibility of P.corylifolia-S.chinensis can alleviate P.corylifolia-induced oxidative damage and endoplasmic reticulum stress of L02 cells.

关 键 词：五味子 补骨脂 配伍减毒 肝损伤 氧化损伤 内质网应激 

分 类 号：R965[医药卫生—药理学]