洋葱总皂苷含量测定条件优化及其体外抗肿瘤活性研究  

Optimization of Content Determination of Total Saponins in Allium Cepa and Its in Vitro Antitumor Activity

作　　者：樊孔明 蔡仁明 杨璐菡 姚秋艳 张世鹏[1] 林勇 杨春艳 FAN Kongming;CAI Renming;YANG Luhan;YAO Qiuyan;ZHANG Shipeng;LIN Yong;YANG Chunyan(School of Pharmacy,North Sichuan Medical College·Institute of Material Medica,North Sichuan Medical College,Nanchong,Sichuan,China 637100;Innovative Platform of Basic Medical Sciences,North Sichuan Medical College,Nanchong,Sichuan,China 637100;Institute of Morphology,North Sichuan Medical College,Nanchong,Sichuan,China 637000;Department of Clinical Medicine,North Sichuan Medical College,Nanchong,Sichuan,China 637000;School of Public Health,North Sichuan Medical College,Nanchong,Sichuan,China 637100)

机构地区：[1]川北医学院药学院·川北医学院药物研究所,四川南充637100 [2]川北医学院基础医学创新研究平台,四川南充637100 [3]川北医学院形态研究所,四川南充637000 [4]川北医学院临床医学系,四川南充637000 [5]川北医学院公共卫生学院,四川南充637100

出　　处：《中国药业》2022年第9期50-54,共5页China Pharmaceuticals

基　　金：2019年四川省大学生创新创业训练计划项目[S201910634029,S201910634079];四川省南充市市校战略合作科研专项[18SXHZ0254,18SXGHZ0214];2021年度名老中医医案研究中心科研项目[YAZX21-YB-41];2020年川北医学院药学一流学科群建设项目[CBY20-YLXK03];川北医学院纵向科研项目[E1QW050301]。

摘　　要：目的优化洋葱醇提物中总皂苷含量测定的显色条件,并评价抗肿瘤活性。方法采用单因素试验优选洋葱醇提物中总皂苷的显色条件,采用香草醛-硫酸比色法测定其含量,采用CCK-8试剂盒测定其对HepG2细胞、HeLa细胞、A549细胞的抑制作用。结果洋葱醇提物中总皂苷的最佳显色条件为使用5%香草醛-冰醋酸0.4 mL,80%硫酸0.6 mL,75℃下反应15 min,冰浴20 min;洋葱醇提物作用24 h后对HepG2细胞、HeLa细胞、A549细胞的半数抑制浓度(IC50)分别为4.463,7.126,3.964 mg/mL,作用48 h后对上述3种细胞的IC_(50)分别为3.371,5.551,8.988 mg/mL。结论该显色条件可客观评价洋葱醇提物中总皂苷的含量,且洋葱醇提物具有抑制HepG2细胞、Hela细胞、A549细胞生长的作用。Objective To optimize the chromogenic conditions for the determination of total saponins in the ethanol extract from Allium cepa,and to evaluate its antitumor activity.Methods The chromogenic conditions of total saponins in the ethanol extract from Allium cepa were optimized by the single-factor test,the content of total saponins in the ethanol extract from Allium cepa was determined by vanillin-sulfuric acid colorimetry method,and the inhibitory effects of the ethanol extract from Allium cepa on the HepG2 cells,HeLa cells and A549 cells were determined by cell counting kit-8(CCK-8).Results The optimal chromogenic conditions of total saponins in the ethanol extract from Allium cepa were as follows:using 0.4 mL of 5%vanillin-glacial acetic acid and 0.6 mL of 80%sulfuric acid,reaction at 75℃for 15 min and ice bath for 20 min.The half-maximal inhibitory concentration(IC50)of the ethanol extract from Allium cepa on the HepG2 cells,HeLa cells and A549 cells after 24 h was 4.463,7.126,3.964 mg/mL,the IC50 of the ethanol extract from Allium cepa on the above three kinds of cells after 48 h was 3.371,5.551,8.988 mg/mL.Conclusion The chromogenic conditions can objectively evaluate the content of total saponins in the ethanol extract from Allium cepa,and the ethanol extract from Allium cepa can inhibit the growth of HepG2 cells,Hela cells and A549 cells.

关 键 词：洋葱 总皂苷 含量测定 单因素试验 抗肿瘤活性 HepG2细胞 HELA细胞 A549细胞 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]