皖南地区MTHFR和PAI-1基因多态性分布及与不良妊娠相关性研究  

Distribution of MTHFR and PAI-1 Gene Polymorphisms and Their Correlation with Adverse Pregnancy in Southern Anhui

作　　者：叶鸣[1] 方佳[1] 赵磊 爱琳 汪思敏 朱和平[1] YE Ming;FANG Jia;ZHAO Lei;AI Lin;WANG Simin;ZHU Heping(Wuhu Hospital of Traditional Chinese Medicine,Wuhu,Anhui,China 241000)

机构地区：[1]安徽省芜湖市中医医院,安徽芜湖241000

出　　处：《中国药业》2022年第9期65-68,共4页China Pharmaceuticals

基　　金：2020年安徽省高校自然科学研究项目[KJ2020A0871]。

摘　　要：目的探讨皖南地区育龄妇女5,10-亚甲基四氢叶酸还原酶(MTHFR)C677T和MTHFR A1298C、血浆纤溶酶原激活物抑制剂-1(PAI-1)基因多态性的分布特征,以及与不良妊娠的相关性。方法选取医院2020年10月至2021年8月收治的育龄妇女122例,其中以61例有不良妊娠史女性为研究组,以61例无不良妊娠史女性为正常组。采用数字荧光分子杂交技术进行MTHFR和PAI-1基因分型,进行组间比较,并与已报道的其他地区进行比较。结果与天津、宁夏比较,皖南地区的PAI-1基因多态性无显著差异(P>0.05);与廊坊比较,MTHFR酶活性低风险、中风险数有显著差异(P<0.05);与贵州比较,MTHFR酶活性正常、中风险数有显著差异(P<0.05)。MTHFR酶活性正常占比由高到低依次为贵州(24.15%)、皖南(8.20%)、廊坊(7.48%);研究组与正常组比较,MTHFR C677T CC和TT基因型,PAI-14G4G和5G5G基因型分布有显著差异(P<0.05),MTHFR A1298C无显著差异(P>0.05)。结论皖南地区育龄妇女的叶酸利用风险较廊坊低,较贵州高;PAI-1基因多态性无地区差异。MTHFR C677T和PAI-1基因多态性与不良妊娠有相关性,MTHFR A1298C与不良妊娠相关性较小。Objective To investigate the distribution characteristics of 5,10-methylenetetrahydrofolate reductase(MTHFR)C677T,MTHFR A1298C and plasma plasminogen activator inhibitor-1(PAI-1)gene polymorphisms and their correlation with the adverse pregnancy in Southern Anhui.Methods A total of 122 childbearing-aged women admitted to the hospital from October 2020 to August 2021 were selected,including 61 women with a history of adverse pregnancy as the study group and 61 women without a history of adverse pregnancy as the normal group.MTHFR and PAI-1 genotypes were analyzed by the digital fluorescence molecular hybridization(DFMH)to conduct the between-group comparisons and comparisons with those reported in other regions.Results Compared with those in Tianjin and Ningxia,there was no significant difference in PAI-1 gene polymorphism in Southern Anhui(P>0.05).Compared with those in Langfang,there was a significant difference in the proportion of low-risk and medium-risk MTHFR enzyme activity(P<0.05).Compared with those in Guizhou,there was a significant difference in the proportions of normal and medium-risk MTHFR enzyme activity.The highest proportion of normal MTHFR enzyme activity was Guizhou(24.15%),followed by Southern Anhui(8.20%)and Langfang(7.48%).There was a significant difference in the distribution of MTHFR C677T CC and TT genotypes,and PAI-14G4G and 5G5G genotypes between the study group and the normal group(P<0.05),while there was no significant difference in MTHFR A1298C between the study group and the normal group(P>0.05).Conclusion The risk of folic acid utilization of childbearing-aged women in Southern Anhui is lower than that in Langfang,while it is higher than that in Guizhou.There was no regional difference in PAI-1 gene polymorphism.MTHFR C677T and PAI-1 gene polymorphisms were correlated with the adverse pregnancy,while MTHFR A1298C was less correlated with the adverse pregnancy.

关 键 词：5 10-亚甲基四氢叶酸还原酶 血浆纤溶酶原激活物抑制剂-1 基因多态性 不良妊娠 相关性 皖南地区 

分 类 号：R95[医药卫生—药学]