黏膜重塑相关因子Wnt1、糖原合成酶激酶-3β、β-连环蛋白在不同类型慢性鼻窦炎中的表达  

Expression of Mucosal Remodeling Related Factors Wnt1 ,Glycogen Synthase Kinase-3 β and β-Catenin in Different Types of Chronic Rhinosinusitis

作　　者：乔新杰 赵玉林[1] 董栋[1] QIAO Xinjie;ZHAO Yulin;DONG Dong(Department of Rhinology,the First Affiliated hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院鼻科,河南郑州450052

出　　处：《河南医学研究》2022年第8期1359-1364,共6页Henan Medical Research

基　　金：国家自然科学基金(82071023)。

摘　　要：目的探讨鼻黏膜组织重塑相关因子Wnt1、糖原合成酶激酶-3β(GSK-3β)、β-连环蛋白(β-catenin)在慢性鼻窦炎(CRS)中的表达。方法本研究采用病例对照研究设计,纳入2020年12月至2021年3月在郑州大学第一附属医院鼻科接受鼻内镜手术的117例患者,分为对照组、慢性鼻窦炎伴鼻息肉(CRSwNP)组和慢性鼻窦炎不伴鼻息肉(CRSsNP)组。对照组取鼻中隔偏曲伴下鼻甲肥大患者的下鼻甲黏膜,CRSwNP组取CRSwNP患者鼻息肉组织,CRSsNP组取CRSsNP患者钩突黏膜。采用苏木精-伊红(HE)染色、糖原染色、马松染色观察鼻黏膜上皮损伤及黏膜上皮重塑情况。采用免疫组织化学法定位并定性检测Wnt1、GSK-3β、β-catenin的表达情况。采用实时荧光定量聚合酶链反应(qPCR)检测Wnt1、GSK-3β、β-catenin在转录水平的表达情况。结果与对照组相比,CRSwNP组和CRSsNP组出现上皮间质转化的病理特点。免疫组织化学结果提示,Wnt1和GSK-3β主要在鼻黏膜上皮细胞胞质中表达;β-catenin在对照组鼻黏膜中主要在细胞质中表达,在CRSwNP组和CRSsNP组中出现细胞质向细胞核中转位的现象;与对照组相比,CRSwNP组和CRSsNP组中Wnt1、β-catenin表达量均增加(P<0.05),且在CRSsNP组中更高(P<0.05);GSK-3β在CRSwNP组和CRSsNP组中的表达量较对照组下降(P<0.05),其中CRSsNP组表达量最低(P<0.05)。在转录水平上,与对照组相比,CRSwNP组和CRSsNP组的Wnt1表达量增加(P<0.05),CRSwNP组中的表达量更高(P<0.05);CRSwNP组和CRSsNP组的GSK-3β、β-catenin表达量较对照组下降(P<0.05),其中CRSsNP组下降更明显。结论Wnt1在CRSsNP和CRSwNP鼻黏膜组织中表达量增加,GSK-3β在CRSsNP和CRSwNP鼻黏膜组织中表达量减少,β-catenin在转录水平及蛋白表达的不一致可能与蛋白的修饰有关。本研究提示鼻黏膜上皮间质转化过程中可能激活了Wnt/β-catenin信号转导通路中的重要因子,并进一步促进CRS鼻黏膜组织重塑。Objective To investigate the expression of nasal mucosal remodeling related factors Wnt1,glycogen synthase kinase-3β(GSK-3β)and β-catenin in chronic rhinosinusitis.Methods A total of 117 patients who underwent endoscopic nasal surgery in the Department of Rhinology of the First Affiliated hospital of Zhengzhou university from December 2020 to March 2021 were enrolled in a case-control design.The subjects were divided into a control group,chronic rhinosinusitis with nasal polyps(CRSwNP)group and chronic rhinosinusitis without nasal polyps(CRSsNP)group.The control group collected inferior turbinate mucosa from nasal septum deviated with inferior turbinate hypertrophy.Nasal polyp tissues from CRSwNP patients were collected from CRSwNP group,and uncinate mucous membranes from CRSsNP patients were collected from CRSsNP group.Hematoxylin-eosin(HE)staining,glycogen staining and Masson staining were used to observe the injury and remodeling of nasal mucosa epithelium.The expression of Wnt1,GSK-3β and β-catenin was located and qualitatively detected by immunohistochemistry.Quantitative real-time polymerase chain reaction(qPCR)was used to detect the expression of Wnt1,GSK-3β and β-catenin at the transcriptional level.Results Compared with the control group,the pathological features of epithelial mesenchymal transformation were observed in the CRSwNP and CRSsNP groups.Immunohistochemical results showed that Wnt1 and GSK-3β were mainly expressed in the cytoplasm of nasal epithelial cells.In the control group,β-catenin was mainly expressed in the cytoplasm,while β-catenin was transferred from the cytoplasm to the nucleus in the CRSwNP and CRSsNP groups.Compared with the control group,the expressions of Wnt1 and β-catenin in CRSwNP and CRSsNP groups were increased(p<0.05),and it was higher in the CRSsNP group(p<0.05).The expression of GSK-3β in and CRSsNP group were lower than that in control group(p<0.05),and the expression of GSK-3β in CRSsNP group was the lowest(P<0.05).At the transcriptional level,compared wit

关 键 词：慢性鼻窦炎 组织重塑 WNT/Β-CATENIN信号转导通路 

分 类 号：R765.41[医药卫生—耳鼻咽喉科]