HPLC-DAD-ELSD联用法同时测定华佗救心丸中13种成分的含量  

Simultaneous Determination of 13 Components in HuatuoJiuxin Pills by HPLC-DAD-ELSD

作　　者：朱雅琴 赵冰 祝宇龙 闫富龙 张云静 吴德玲 彭灿 吴欢 ZHU Ya-qin;ZHAO Bing;ZHU Yu-long;YAN Fu-long;ZHANG Yun-jing;WU De-ling;PENG Can;WU Huan(School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;Anhui Province Key Laboratory of Pharmaceutical Preparation Technology and Application,Hefei 230012,China;Anhui Key Laboratory of Chinese Medicinal Formula,Hefei 230012;Huatuo Medicines Co.,Ltd.,Bozhou 236800,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]药物制剂技术与应用安徽省重点实验室,安徽合肥230012 [3]中药复方安徽省重点实验室,安徽合肥230012 [4]华佗国药股份有限公司,安徽亳州236800

出　　处：《化学试剂》2022年第5期762-766,共5页Chemical Reagents

基　　金：安徽省科技重大专项项目(18030801117);安徽省企业联合发展基金项目(RD19200008);安徽省高等学校自然科学研究项目(KJ2019A0465);安徽省高校优秀人才支持计划项目(gxyqZD2020025)。

摘　　要：建立一种快速的HPLC-DAD-ELSD联用方法,同时测定华佗救心丸中皂苷类、蟾蜍甾烯类以及胆酸类13种成分的含量。采用HPLC-DAD-ELSD联用,色谱柱Caprisil C18(250 mm×4.6 mm,5μm),柱温35℃,并以水和乙腈作为流动相进行梯度洗脱。紫外检测波长为296 nm和203 nm,ELSD检测参数:漂移管温度60℃、气体压力241 kPa,检测时间为18 min。华佗救心丸中的13种分析成分线性r值均大于0.99,线性关系良好(r≥0.99),平均加样回收率在96.31%~103.30%范围内,RSD值为1.42%~2.75%。该方法更快速全面的同时测定华佗救心丸中13种分析成分含量,专属性强、精确度高,可为华佗救心丸质量控制和进一步的品质提升提供参考。The HPLC-DAD-ELSD method was established to simultaneously determine the contents of 13 saponins,bufadienolides and bile acids in HuatuoJiuxin Pills.The established HPLC-DAD-ELSD method was adopted,in which a Caprisil C18 column(250 mm×4.6 mm,5μm)was used,the column temperature was 35℃,and water-acetonitrile was used as the mobile phase for gradient elution.In addition,the wavelength of UV detection was 296 nm and 203 nm.The temperature of the drift tube in the detection parameters of the evaporative light scattering detector(ELSD)was 60℃,the gas pressure was 241 kPa and the gain was 100.Finally,the total detection time was 18 min.The linear r values of the 13 analytical components in HuatuoJiuxin Pills were all greater than 0.99,so that the linear relationship was well(r≥0.99).The average recovery rate of sample addition was in the range of 96.31%to 103.30%,and the RSD was 1.42%to 2.75%.This method can simultaneously determine the content of 13 analytical components in Huatuo Jiuxin Pills more quickly and comprehensively,with strong specificity and high accuracy,which can provide references for the quality control and further quality improvement of HuatuoJiuxin Pills.

关 键 词：华佗救心丸 高效液相色谱法-全波长检测-蒸发光散射器联用 蟾蜍甾烯类 胆酸类 皂苷类 

分 类 号：R284.1[医药卫生—中药学]