黄芩素在七氟醚诱导的多巴胺神经元损伤中的作用及机制  

Role and mechanism of baicalein in sevoflurane-induced dopamine neuron injury

作　　者：赵品 蒯建科 杨倩[1] 张宏[1] 高龙飞 张徽 ZHAO Pin;KUAI Jianke;YANG Qian;ZHANG Hong;GAO Longfei;ZHANG Hui(Department of Anesthesiology,Xi'an No.3 Hospital,the Affiliated Hospital of Northwest University,Xi'an 710018,China)

机构地区：[1]西北大学附属医院·西安市第三医院麻醉科,陕西西安710018

出　　处：《临床医学研究与实践》2022年第14期5-9,共5页Clinical Research and Practice

摘　　要：目的探讨黄芩素在七氟醚诱导的多巴胺神经元损伤中的作用及机制。方法使用黄芩素预处理SN4741细胞1 h,再使用0%、1%、3%、5%的七氟醚分别处理12 h,采用相应方法检测SN4741细胞活性及Fe^(2+)、ROS、GSH、GPX4蛋白水平。结果与0%七氟醚处理12 h比较,1%七氟醚处理12 h SN4741细胞活性无明显变化(P>0.05),3%、5%七氟醚处理12 h SN4741细胞活性显著降低(P<0.05)。与对照组比较,七氟醚组SN4741细胞Fe^(2+)、ROS水平显著升高,SN4741细胞GSH水平显著降低(P<0.05);与七氟醚组比较,七氟醚+黄芩素组SN4741细胞Fe^(2+)、ROS水平显著降低,SN4741细胞GSH水平显著升高(P<0.05)。与七氟醚组比较,七氟醚+黄芩素10μM组、七氟醚+黄芩素20μM组SN4741细胞活性显著升高(P<0.05)。与对照组比较,七氟醚组SN4741细胞GPX4蛋白水平显著降低(P<0.05);与七氟醚组比较,七氟醚+黄芩素组SN4741细胞GPX4蛋白水平显著升高(P<0.05)。结论随着七氟醚浓度的升高,SN4741细胞活性逐渐降低。七氟醚处理可导致SN4741细胞Fe^(2+)、ROS水平升高,GSH水平降低,而黄芩素预处理可以逆转Fe^(2+)、ROS及GSH水平的变化,减少七氟醚引起的铁死亡。黄芩素还可通过升高GPX4蛋白水平来减轻七氟醚诱导的SN4741细胞铁死亡。Objective To investigate the role and mechanism of baicalein in sevoflurane-induced dopamine neuron injury.Methods SN4741 cells were pretreated with baicalein for 1 h and then treated with 0%,1%,3%and 5%sevoflurane for 12 h respectively.The activity of SN4741 cells and the levels of Fe^(2+),ROS,GSH and GPX4 protein were detected by corresponding methods.Results Compared with 0%sevoflurane treated for 12 h,the activity of SN4741 cells treated with 1%sevoflurane for 12 h did not change significantly(P>0.05),and the activity of SN4741 cells treated with 3%and 5%sevoflurane for 12 h decreased significantly(P<0.05).Compared with the control group,the levels of Fe^(2+) and ROS in SN4741 cells of the sevoflurane group increased significantly,and the level of GSH in SN4741 cells decreased significantly(P<0.05);compared with the sevoflurane group,the levels of Fe^(2+) and ROS in SN4741 cells of the sevoflurane+baicalein group decreased significantly,and the level of GSH in SN4741 cells increased significantly(P<0.05).Compared with the sevoflurane group,the activity of SN4741 cells in the sevoflurane+baicalein 10μM group and the sevoflurane+baicalein 20μM group increased significantly(P<0.05).Compared with the control group,the level of GPX4 protein in SN4741 cells of the sevoflurane group decreased significantly(P<0.05);compared with the sevoflurane group,the level of GPX4 protein in SN4741 cells of the sevoflurane+baicalein group increased significantly(P<0.05).Conclusion With the increase of sevoflurane concentration,the activity of SN4741 cells decreased gradually.Sevoflurane treatment can increase the levels of Fe2+and ROS and reduce the level of GSH in SN4741 cells,while baicalein pretreatment can reverse the changes of Fe2+,ROS and GSH levels and reduce the iron death caused by sevoflurane.Baicalein can also reduce the sevoflurane-induced iron death of SN4741 cells through increasing the level of GPX4 protein.

关 键 词：黄芩素 七氟醚 多巴胺神经元损伤 铁死亡 

分 类 号：R69[医药卫生—泌尿科学]