冬青卫矛LTR反转录转座子RT序列克隆及分析  

Cloning and Analysis of RT Sequences of LTR Retrotransposons in Euonymus japonicus

作　　者：贺诗琪 范付华 吴宇航[1,2] 龙蓉 王君荣 陈美吉 He Shiqi;Fan Fuhua;Wu Yuhang;Long Rong;Wang Junrong;Chen Meiji(Institute for Forest Resources and Environmental of Guizhou/Key Laboratory of Forest Cultivation in Plateau Mountain of Guizhou Province,Guizhou University,Guiyang,Guizhou 550025,China;College of Forestry,Guizhou University,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州大学贵州省森林资源与环境研究中心/贵州省高原山地林木培育重点实验室,贵州贵阳550025 [2]贵州大学林学院,贵州贵阳550025

出　　处：《山地农业生物学报》2022年第3期1-6,27,共7页Journal of Mountain Agriculture and Biology

基　　金：贵州省一流学科建设项目(GNY[2017]007);贵州大学大学生“SRT计划”项目(贵大SRT字[2019]266)。

摘　　要：本文以冬青卫矛基因组DNA为模板,克隆LTR反转录转座子Ty1-copia类与Ty3-gypsy类反转录酶(reverse transcriptase,RT)序列,通过测序及相关生物信息学软件对所获序列变化特点进行分析。最终获得Ty1-copia与Ty3-gypsy类反转录转座子RT序列分别为40条和39条。其中Ty1-copia-RT序列长度在231~267 bp之间,核酸序列的相似性为8.05%~97.7%;Ty3-gypsy-RT序列长度范围为365~499 bp,核酸序列的相似性为10.04%~99.31%。将所获得的核酸序列翻译为氨基酸后发现RT氨基酸序列具有高度异质性。通过与其他物种RT序列进行比对,结果显示冬青卫矛与其他一些植物间的部分RT序列相似性较高,可能存在共同起源,印证了LTR反转录转座子不同物种间可能存在横向传递。In this paper,reverse transcriptase(RT) sequences of Ty1-copia and Ty3-gypsy retrotransposons were obtained through PCR amplification using Euonymus japonicus genomic DNA as template.The obtained sequences were analyzed by sequencing and bioinformatics software.Results revealed that 40 Ty1-copia like RT sequences and 39 Ty3-gypsy like RT sequences were identified.The length of Ty1-copia-RT sequences ranged from 231 bp to 267 bp,and the similarity of nucleic acid sequences ranged from 8.05% to 97.7%.The length of Ty3-gypsy-RT sequences was from 365 bp to 499 bp,and the similarity of nucleic acid sequences ranged from 10.04% to 99.31%.The translated RT amino acid sequences showed high heterogeneity.The phylogenetic analysis of RT sequences showed diverse similarity between E.japonicus and other species,reflecting that they had a common origin,and a horizontal transmission of LTR retrotransposons occurred in the process of evolution among different species.

关 键 词：冬青卫矛 LTR反转录转座子 反转录酶 异质性 

分 类 号：S718.3[农业科学—林学]