强舒降压方对L-NAME诱导大鼠高血压降压作用及氧化应激机制研究  

Effect of Qiangshu Jiangya Formula on L-NAME-induced Hypertension and Oxidative Stress in Rats

作　　者：黄自通 黄晓霞 赵璐 杨伟韬 王红刚 Huang Zitong;Huang Xiaoxia;Zhao Lu;Yang Weitao;Wang Honggang(Qingyuan Polytechnic College of Food and Drugs,Qingyuan 511510;Guangdong Pharmaceutical University College of Traditional Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]清远职业技术学院食品药品学院,广东清远511510 [2]广东药科大学中药学院,广东广州510006

出　　处：《广东化工》2022年第8期51-54,38,共5页Guangdong Chemical Industry

基　　金：广东省高水平专业群建设(490201);广东省粤北中药材深加工工程技术研究中心,负责人:黄自通。

摘　　要：目的:阐明强舒降压方(QJF)对大鼠高血压降压作用及氧化应激机制。方法:采用L-NAME诱导高血压模型,设立空白组、模型组、卡托普利组、QJF高、中、低剂量组;连续灌胃给药10周,每周测量大鼠血压1次,末次给药1h后测量血压;腹腔注射麻醉,取材。HE染色观察胸主动脉、肾脏、心脏病理变化;测量血浆GSH(还原型谷胱甘肽)含量,血清SOD(超氧化物歧化酶)含量,尿蛋白浓度,肝脏匀浆MDA(丙二醛)、蛋白质羰基含量。结果:与模型组相比,QJF低、中、高剂量组与卡托普利组收缩压、舒张压显著降低(P<0.05或P<0.01),QJF低剂量组和卡托普利组血清SOD、血浆GSH均显著升高(P<0.05),尿蛋白浓度、肝脏MDA含量与蛋白质羰基显著降低(P<0.05或P<0.01);组织病理学检查显示,QJF干预后胸主动脉、肾脏、心脏病理改善明显。结论:QJF对L-NAME诱导的大鼠高血压具有降压作用,其降压机制可能与GSH、SOD、尿蛋白、MDA、蛋白质羰基等氧化应激指标有关,并对胸主动脉、肾脏、心脏组织病理具有改善作用。Objective: To clarify the mechanism of Qiangshu Jiangya Decoction(QJF) on hypertension and oxidative stress in rats. Methods: The hypertension model was induced by L-NAME. The blank group, model group, captopril group and QJF high, medium and low dose groups were established. After intragastric administration for 10 weeks, the blood pressure of rats was measured once a week, and the blood pressure was measured 1 h after the last administration. Anesthesia by intraperitoneal injection. HE staining was used to observe the pathological changes of thoracic aorta, kidney and heart;plasma GSH(reduced glutathione) content,serum SOD(superoxide dismutase) content, urinary protein concentration, liver homogenate MDA(malondialdehyde), protein carbonyl content were measured.Results: Compared with the model group, the systolic blood pressure and diastolic blood pressure of QJF low, medium and high dose group and captopril group were significantly decreased(P<0.05 or P<0.01). The serum SOD and plasma GSH of QJF low dose group and captopril group were significantly increased(P<0.05).The urinary protein concentration, liver MDA content and protein carbonyl were significantly decreased( P<0.05 or P<0.01). Histopathological examination showed that the thoracic aorta, kidney and heart disease were significantly improved after QJF intervention. Conclusion: QJF has a antihypertensive effect on L-NAME-induced hypertension in rats, and its antihypertensive mechanism may be related to oxidative stress indexes such as GSH, SOD, urinary protein, MDA and protein carbonyl, and can improve the histopathology of thoracic aorta, kidney and heart.

关 键 词：强舒降压方 L-NAME 血压 氧化应激 病理 

分 类 号：R285.5[医药卫生—中药学]