普拉梭菌对溃疡性结肠炎不典型增生患者Treg细胞水平及IL-23/IL-17轴的影响  

The effect of Faecalibacteriumprausnitzii on Treg cell levels and IL-23/IL-17axis in patients with dysplasia of ulcerative colitis

作　　者：张琳[1,2] 魏凯艳 刘益娟 ZHANG Lin;WEI Kai-yan;LIU Yi-juan(Department of Gastrointestinal Surgery,the First Affiliated Hospital of Fujian Medical University,Fuzhou,Fujian 350002,China;The First Clinical Medical College of Fujian Medical Universit;Department of Gastroenterology,the First Affiliated Hospital of Fujian Medical Universityy)

机构地区：[1]福建医科大学附属第一医院胃肠外科,福建福州350002 [2]福建医科大学第一临床医学院 [3]福建医科大学附属第一医院消化内科

出　　处：《中国病原生物学杂志》2022年第2期224-228,共5页Journal of Pathogen Biology

摘　　要：目的研究普拉梭菌(Faecalibacteriumprausnitzii)对溃疡性结肠炎(ulcerative colitis,UC)不典型增生患者调节性T细胞(Treg)水平及白介素23(IL-23)/白介素17(IL-17)轴的影响。方法收集25例UC不典型增生患者和20例健康对照者的新鲜粪便以及UC不典型增生患者病变、正常肠黏膜组织。提取新鲜粪便中的细菌基因组DNA,采用实时荧光定量PCR检测普拉梭菌基因拷贝数,采用免疫组化和Western blot检测组织IL-17和IL-23表达水平,采用流式细胞术检测全血Treg细胞水平,比较不同组受试者普拉梭菌含量以及Treg、IL-23、IL-17的差异性,采用Pearson相关分析普拉梭菌含量与Treg细胞水平及IL-23/IL-17的关系。结果UC不典型增生组患者新鲜粪便普拉梭菌含量以及全血Treg细胞水平均显著高于对照组(均P<0.01);病变肠黏膜组织IL-17和IL-23表达水平均显著高于正常组织(P<0.01)。Pearson分析显示,粪便中普拉梭菌含量与外周血Treg细胞水平呈正相关性(r=0.526,P<0.01),而与组织中的IL-23、IL-17水平呈负相关性(r值分别为-0.474和-0.487,均P<0.01)。结论普拉梭菌可改善UC不典型增生患者结肠组织病理学状态,其机制可能为促进Treg细胞的表达以及抑制IL-23/IL-17轴。Objective To study the effect of Faecalibacteriumprausnitzii on the levels of regulatory T cells(Treg)and interleukin 23(IL-23)/interleukin 17(IL-17)axis.Methods The fresh stools of 25patients with UC dysplasia and 20healthy controls,as well as the diseased and normal intestinal mucosal tissues of patients with UC dysplasia were collected.The bacterial genomic DNA from fresh feces was extracted.The real-time fluorescent quantitative PCR was used to detect gene copy number of Clostridium pruritus.The immunohistochemistry and western blot were used to detect the expression levels of IL-17and IL-23in tissues.The flow cytometry was used to detect whole blood Treg cell level.The content of Clostridium praevia,Treg cell,IL-23and IL-17level in different groups were compared.Pearson correlation was used to analyze the relationship between the content of Clostridium praevia and Treg cell and IL-23/IL-17level.Results The content of Clostridium prasatoid and the level of Treg cells in whole blood in the fresh feces of patients with UC dysplasia group were significantly higher than those of the control group(all P<0.01);the expression levels of IL-17and IL-23in the diseased intestinal mucosa were significantly higher those in the normal tissues(P<0.01).Pearson analysis showed that the content of Clostridium prasmodium in feces had a positive correlation with the level of peripheral blood Treg cells(r=0.526,P<0.01),and a negative correlation with the levels of IL-23and IL-17in the tissues(r value=-0.474and-0.487,respectively,both P<0.01).Conclusion Clostridium prastilinoids can improve the histopathological state of colon in patients with UC dysplasia.The mechanism may be to promote the expression of Treg cells and inhibit the IL-23/IL-17axis.

关 键 词：溃疡性结肠炎 普拉梭菌 调节性T细胞 白介素23 白介素17 

分 类 号：R378[医药卫生—病原生物学]