夏枯草对果糖诱导的大鼠代谢综合征的影响及其代谢机制  

Effect and Mechanism of Prunellae Spica on Metabolic Syndrome in Rats

作　　者：汤杨黔南 陈梓玉 李利 杨盾 王枭冶 谢明霞[1,2] TANG Yang-qian-nan;CHEN Zi-yu;LI Li;YANG Dun;WANG Xiao-ye;XIE Ming-xia(Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Brain Hospital(The Second People's Hospital of Hunan Province),Changsha 410007,China)

机构地区：[1]湖南中医药大学,长沙410208 [2]湖南省脑科医院(湖南省第二人民医院),长沙410007

出　　处：《中国实验方剂学杂志》2022年第9期125-132,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国博士后科学基金第69批面上资助项目(2021M690990);湖南省自然科学基金科药联合项目(2021JJ80060);湖南省卫生健康委员会项目(202107021634)。

摘　　要：目的:代谢综合征是多种疾病的表现形式,严重危害心脑血管系统。中药夏枯草能调节高脂小鼠脂代谢紊乱状态,有效抑制肝损伤代谢紊乱,针对夏枯草对代谢综合征的影响及代谢机制进行分析,试图从天然产物中寻找潜在的安全药物。方法:采用果糖诱导复制大鼠代谢综合征模型,基于气相色谱-质谱联用仪(GC-MS)的方法探讨夏枯草提取物对代谢综合征大鼠的作用及可能的代谢机制。结果:夏枯草提取物可显著降低代谢综合征大鼠体质量,降低血脂水平,抗脂质过氧化损伤,抑制血清肿瘤坏死因子(TNF)-α炎症因子的释放,保护肝脏维护其正常功能运行。多元统计分析结果显示,代谢综合征大鼠血清中代谢物发生显著变化,夏枯草处理可显著改善其变化。与正常组比较,代谢综合征大鼠中发现11个差异代谢标志物;与模型组比较,夏枯草组中有8个显著差异代谢标志物,其中,磷酸盐、丙酮酸和琥珀酸为共有的标志物。通路分析显示,夏枯草的调节作用主要与三羧酸循环、糖酵解和糖异生、丝氨酸/苏氨酸和甘氨酸代谢途径有关。结论:夏枯草可作为治疗代谢综合征潜在的天然药物来源,其可通过能量代谢和氨基酸代谢途径调节果糖诱导的代谢综合征的代谢紊乱。Objective:Metabolic syndrome is the inherent phenotype of many diseases,which seriously endangers the cardio-cerebrovascular system.Prunellae Spica can regulate lipid metabolism disorder in high-fat mice and inhibit the metabolic disorder of liver injury.This study analyzed the effect of Prunellae Spica on metabolic syndrome and its mechanism,and it is of great significance to find potential safe drugs from natural products.Method:In this study,the metabolic syndrome model was induced by fructose.The metabolomics method based on gas chromatography-mass spectrometry(GC-MS)was used to explore the effect and mechanism of Prunellae Spica on rats with metabolic syndrome.Result:Pharmacological results showed that Prunellae Spica significantly reduced the body weight,blood lipid level and lipid peroxidation level and inhibited the release of tumor necrosis factor-α(TNF-α)in rats with metabolic syndrome.Thus,Prunellae Spica protected the liver and maintained its normal functions.Multivariate statistical analysis revealed that metabolites in the serum of rats with metabolic syndrome changed significantly,which was improved after Prunellae Spica treatment.Compared with the metabolites in normal group,11 differential metabolic markers were found in rats with metabolic syndrome.Compared with model group,Prunellae Spica group had 8 significantly different metabolic markers,among which phosphate,pyruvic acid and succinic acid were common markers.Pathway analysis indicated that the regulatory effect of Prunellae Spica was mainly related to citrate cycle,glycolysis and gluconeogenesis,serine/threonine and glycine metabolic pathways.Conclusion:Prunellae Spica can be used as a potential natural source for the treatment of metabolic syndrome.It can regulate the metabolic disorder in metabolic syndrome via energy and amino acid metabolism.

关 键 词：夏枯草 果糖诱导 代谢综合征 代谢组学 气相色谱-质谱联用仪 

分 类 号：R284.2[医药卫生—中药学] R285[医药卫生—中医学]