两种纳米材料对纤毛虫绿草履虫超微结构的影响  

The effects of two nanomaterials on the ultrastructure of Paramecium bursaria

作　　者：谭炳钰 巩志伟 王奕文[1] 倪兵[1] TAN Bing-yu;GONG Zhi-wei;WANG Yi-wen;NI Bing(School of Life Sciences,East China Normal University,Shanghai200241;School of Physics and Electronic Science,East China Normal University,Shanghai200241,China)

机构地区：[1]华东师范大学生命科学学院,上海200241 [2]华东师范大学物理与电子科学学院,上海200241

出　　处：《电子显微学报》2022年第2期161-170,共10页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目(No.31672249)。

摘　　要：为探究纳米材料进入水环境后对水生生物的影响,本文利用扫描及透射电子显微镜观察了纳米银和纳米铜对纤毛虫绿草履虫(Paramecium bursaria)细胞超微结构的损伤情况。结果表明:(1)两种纳米材料对细胞结构的损伤方式具有一定的相似性,损伤主要表现在绿草履虫细胞的表膜、线粒体以及细胞核等结构,且损伤程度随时间增加逐渐加重。(2)纳米铜对细胞超微结构造成的损伤程度比纳米银严重。实验结果表明,两种纳米材料均对纤毛虫具有生物毒性,推测其通过氧化应激导致细胞表面及内部结构损伤。In order to access the effect of nanomaterials on aquatic organisms,the damages of silver and copper nanoparticles on the ultrastructure of Paramecium bursaria were observed by scanning and transmission electron microscopy.The results showed that:(1) The damages of the two nanomaterials to the cell structure were similar,and were mainly manifested in the surface membrane,mitochondria and nucleus of cells.The degree of the damages gradually became stronger with the increase of time.(2) The damage of copper nanoparticles on cell ultrastructure is more serious than that of silver nanoparticles.According to the results,the two nanomaterials had a biological toxicity to ciliates,and the damages on the cell surface as well as on the internal structure could be caused by the two nanomaterials through the oxidative stress.

关 键 词：纳米银 纳米铜 绿草履虫 超微结构 

分 类 号：Q952.4[生物学—动物学] Q958.8