四碘荧光素B介导光动力通过破坏病毒核酸有效灭活包膜RNA病毒的研究  

Study of Erythrosine B-Mediated Photodynamic to Effectively Inactivate Enveloped RNA Viruses by Destroying Viral Nucleic Acid

作　　者：丁晓艳 杨茂成 郭红霞 晁晓娟[2] 李晋涛 DING Xiaoyan;YANG Maocheng;GUO Hongxia;CHAO Xiaojuan;LI Jintao(Department of Military Biosafety,College of Basic Medicine,Army Medical University,Chongqing400038,China)

机构地区：[1]陆军军医大学基础医学院军事生物安全教研室,重庆400038 [2]新疆军区总医院疾病预防控制科

出　　处：《华南国防医学杂志》2022年第2期79-82,共4页Military Medical Journal of South China

基　　金：军队后勤项目(CWS20C008)。

摘　　要：目的研究四碘荧光素B介导光动力对有包膜RNA病毒的消杀效果,评估该消杀方案在破坏病毒核酸的可行性。方法以有包膜的登革病毒4(Dengue virus 4,DENV4)和辛德毕斯病毒(Sindbis virus,SINV)作为指示病毒,添加2μg/ml四碘荧光素B,在可见光波长535 nm下照射10 min,进行灭活处理。观察细胞病变并采用实时荧光定量逆转录聚合酶链式反应(real-time quantitative reverse transcripition polymerase chain reaction,RT-PCR)进行消杀效果评价。结果四碘荧光素B加光照处理DENV4和SINV后,可通过破坏病毒核酸,有效阻止其对细胞的感染。结论四碘荧光素B作为良好的光敏剂与可见光结合可有效灭活有包膜的RNA病毒,可作为体外病毒灭活的新途径。Objective To study the disinfective efficacy of erythrosine B-mediated photodynamic against enveloped RNA viruses and evaluate the feasibility of disinfective scheme in destroying viral nucleic acid. Methods Dengue virus 4(DENV 4) and Sindbis virus(SINV) were used as indicative virus, which were added with 2 μg/ml erythrosine B and illuminated at 535 nm for 10 min. The disinfective effect was evaluated by observing cytopathic disease and real-time quantitative reverse transcripition polymerase chain reaction(RT-PCR). Results Erythrosine B-mediated photodynamic could effectively prevent the infection and replication of DENV 4 and SINV by destroying the viral nucleic acid of the virus. Conclusion Erythrosine B, as a good photosensitizer mediated photodynamics, can effectively inactivate enveloped RNA viruses, and can be used as a new way to hactivate virsuses in vitro.

关 键 词：光动力疗法 四碘荧光素B 包膜RNA病毒 登革病毒4 辛德毕斯病毒 

分 类 号：R187[医药卫生—流行病学]