海洋酸化与陆源DON对米氏凯伦藻和东海原甲藻生长的影响  

The Effect of Ocean Acidification and Land-derived Dissolved Organic Nitrogen on the Growth of Karenia mikimotoi and Prorocentrum donghaiense

作　　者：张艳红 贺云凤 李克强[1] 张现盛 杨锐 逄凯 梁生康[1] 王修林[1] Zhang Yanhong;He Yunfeng;Li Keqiang;Zhang Xiansheng;Yang Rui;Pang Kai;Liang Shengkang;Wang Xiulin(The Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China)

机构地区：[1]中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东青岛266100

出　　处：《中国海洋大学学报（自然科学版）》2022年第5期86-96,共11页Periodical of Ocean University of China

基　　金：国家重点研究发展计划项目(2017YFC1404300);国家自然科学基金项目(41676062);中央高校基本科研业务费专项(201962008);国家基金委-山东省联合基金项目(U1706215);山东省重大科技创新工程项目(2019JZZY020705)资助

摘　　要：本文通过实验室培养实验,研究以生活源溶解有机氮(DON)为氮源和不同水平pCO_(2)(380和800 ppmv)条件下,DON和海洋酸化对东海原甲藻(Prorocentrum donghaiense)和米氏凯伦藻(Karenia mikimotoi)两种典型赤潮甲藻生长和竞争的影响。结果表明,在高pCO_(2)水平下,东海原甲藻和米氏凯伦藻的生长均受到抑制,表现为过氧化氢酶(CAT)或超氧化物歧化酶(SOD)活性增加,而对DON的吸收则影响不大。在以DON为氮源单独培养条件下,东海原甲藻较米氏凯伦藻生长更快,表现为更快的生长速率和更高的DON吸收亲和力指数(V_(max)/K_(s))。在以DON为氮源混合培养条件下,快速生长的东海原甲藻在初始阶段成为优势藻种,而生长较慢的米氏凯伦藻能继续利用环境中DON,在后期演替成为优势种。海洋酸化(pCO_(2):800 ppmv)可能会因米氏凯伦藻的生长受到更大的影响,从而降低了其在赤潮后期与东海原甲藻的竞争优势。本文的研究成果有助于对控制和缓解东海原甲藻和米氏凯伦藻赤潮提供科学依据。In this study, under the condition of ambient DON from domestic sources and different levels of pCO2 (380 ppmv and 800 ppmv), DON and ocean acidification effect on the growth and competition of two typical red tide dinoflagellates (Prorocentrum donghaiense and Karenia mikimotoi) by laboratory research. The results showed that the growth of P. donghaiense and K. mikimotoi were both inhibited under elevated pCO_(2) level, which showed the increased the catalase (CAT) and superoxide dismutase (SOD) activities, while high pCO_(2) level had little effect on DON uptake by these two dinoflagellates. Under the condition of DON as nitrogen source, P. donghaiense grew faster than K. mikimotoi, which was embodied in faster growth rate and higher DON uptake affinity index (V_(max)/K_(s)). Under the condition of mixed culture with DON as nitrogen source, the fast-growing algae of P. donghaiense became the dominant species in the initial stage, while the slower-growing algae of K. mikimotoi could continue to utilize DON in the environment and became the dominant species after the lag phase. Ocean acidification(pCO_(2) 800 ppmv)reduced the competitive advantage of K. mikimotoi at the late stage of red tide with P. donghaiense, due to the growth of K. mikimotoi might be more affected by ocean acidification. The results of our study could provide a scientific basis for red tide of P. donghaiense and K. mikimotoi control and mitigation.

关 键 词：米氏凯伦藻 东海原甲藻 溶解有机氮 海洋酸化 抗氧化酶 

分 类 号：P734[天文地球—海洋化学]