Time Dependent Motility of Stallion Semen during Cool Storage Centrifuged in Two Different Extenders  

作　　者：Christine Stroink-Pickering Yuna Lee Peyton Arnett Kevin Halden Kline 

机构地区：[1]Department of Animal Sciences,University of Illinois at Urbana Champaign,1207 West Gregory Drive,Urbana,IL 61801,USA

出　　处：《Journal of Agricultural Science and Technology(A)》2022年第1期15-21,共7页农业科学与技术（A）

摘　　要：The aim of this study was to evaluate the maintenance of spermatozoa motility over 72 h by two commercial semen extenders used to centrifuge semen before re-suspending the sperm pellet in INRA 96^(■).Centrifuged and re-suspended semen samples were evaluated for percent motility using four commercial Standard bred stallions collected three times each during the spring and summer breeding season.Next Generation Dr.Kenney’s^(■)without antibiotic or INRA 96^(■)semen extenders were used to dilute semen samples in a 1:1 ratio prior to centrifugation at 750×g for 10 min.After decanting the supernatant,the resulting sperm pellets were then re-suspended and extended with INRA 96^(■)to a final concentration of 50 million total cells per millimeter and stored in Equine Express II stallion semen shipping containers.Percent progressive motility was recorded at time points 0,24,48,and 72 h.Analysis of variance between stallions,time,and extender type found that there was no significant difference in percent motility at each of the time points between the two extenders(p=0.87)used for centrifugation.Differences in percent motility over time and between stallions were found(p<0.001).The findings suggest that the use of Next Generation Dr.Kenney’s^(■)semen extender during centrifugation and then re-suspending the sperm pellet in INRA 96^(■)may be more cost effective than using INRA 96^(■)for both centrifugation and re-suspension of the sperm pellet.This is due to the fact that the less expensive Next Generation Dr.Kenney’s^(■)supernatant is decanted and discarded after the centrifugation step,instead of the much more expensive INRA 96^(■),yet does not affect the quality of semen preservation and motility over time.

关 键 词：STORAGE BREEDING MILLIMETER 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]