丹参酮ⅡA联合阿托伐他汀对大鼠动脉粥样硬化的治疗作用研究  

Study of Therapeutic Effects of TanshinoneⅡA Combined with Atorvastatin on Atherosclerosis in Rats

作　　者：叶泽森 周里岩 黄光业 薛照芸[1] 刘培庆[1] 陈健文[1] YE Ze-sen;ZHOU Li-yan;HUANG Guang-ye;XUE Zhao-yun;LIU Pei-qing;CHEN Jian-wen(College of Pharmacy,Sun Yat-sen University,Guangzhou 510006,Guangdong,China)

机构地区：[1]中山大学药学院,中国广东广州510006

出　　处：《生命科学研究》2022年第2期95-102,共8页Life Science Research

基　　金：广东省产学研合作领域项目(2017B090903004);广东省本土创新科研团队项目(2017BT01Y093)。

摘　　要：阿托伐他汀和丹参酮ⅡA在临床上已用于治疗动脉粥样硬化。然而,两者联用治疗动脉粥样硬化的协同作用仍不明确。为了考察丹参酮ⅡA联合阿托伐他汀能否增强阿托伐他汀对动脉粥样硬化大鼠的治疗效果,将大鼠随机分为正常对照组、模型组、丹参酮ⅡA低剂量组(7 mg/kg)、丹参酮ⅡA中剂量组(21 mg/kg)、丹参酮ⅡA高剂量组(63 mg/kg)、阿托伐他汀组(7 mg/kg)、丹参酮ⅡA低剂量(7 mg/kg)+阿托伐他汀(7 mg/kg)组、丹参酮ⅡA中剂量(21 mg/kg)+阿托伐他汀(7 mg/kg)组。除正常对照组外,其余大鼠采用连续4 d腹腔注射300000 IU/(kg·d)维生素D3配合高脂饲料喂养建立动脉粥样硬化模型。造模16周后,检测各组大鼠血清总胆固醇(total cholesterol,TC)、甘油三酯(triacylglycerol,TG)、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)含量;同时,通过苏木精-伊红染色观察腹主动脉病理学变化。结果显示,联合用药组能显著降低动脉粥样硬化大鼠血清中TC、TG、LDL-C含量(P<0.01);高剂量的丹参酮ⅡA和阿托伐他汀均能明显抑制腹主动脉内膜增厚,中剂量的丹参酮ⅡA联合阿托伐他汀组抑制效果最强(P<0.01)。上述结果表明,丹参酮ⅡA联合阿托伐他汀对动脉粥样硬化大鼠具有更好的治疗效果。Atorvastatin and tanshinoneⅡA have been clinically used in the treatment of atherosclerosis,but the synergistic effect of the two is still unclear.To explore whether their combination can enhance the preventive and therapeutic effects of atorvastatin on atherosclerosis in rats,the rats were randomly divided into control group,model group,low(7 mg/kg)-,middle(21 mg/kg)-,and high(63 mg/kg)-dose tanshinoneⅡA groups,atorvastatin group(7 mg/kg),low-dose tanshinoneⅡA(7 mg/kg)+atorvastatin(7 mg/kg)group,and middle-dose tanshinoneⅡA(21 mg/kg)+atorvastatin(7 mg/kg)group.Except for the control group,rats in other groups were intraperitoneal injected with 300000 IU/(kg·d)vitamin D3 for 4 days and fed with highfat diet to establish an atherosclerosis model.After 16 weeks,the concentrations of serum total cholesterol(TC),triacylglycerol(TG),high-density lipoprotein cholesterol(HDL-C),and low-density lipoprotein cholesterol(LDL-C)in rats were determined.In addition,the pathological changes of abdominal aorta were observed by hematoxylin-eosin staining.The results showed that the concentrations of TC,TG,and LDL-C in sera of atherosclerotic rats were significantly decreased in the combination treatment groups(P<0.01).Highdose tanshinoneⅡA and atorvastatin significantly inhibited intimal thickening of the abdominal aorta,and middle-dose tanshinoneⅡA combined with atorvastatin had the strongest inhibition effect(P<0.01).In conclusion,the combination of tanshinoneⅡA with atorvastatin exhibits a better treatment effect on atherosclerosis in rats.

关 键 词：丹参酮ⅡA 阿托伐他汀 联合用药 动脉粥样硬化(AS) 血脂水平 

分 类 号：Q955[生物学—动物学] R543.5[医药卫生—心血管疾病]