不同方法建立长期慢性高尿酸血症模型及对相关并发症影响的研究进展  

Research Progress in Long-term Chronic Hyperuricemia Modeling Methods and Their Impacts on Associated Complications

作　　者：张乐琦 林翠婷 黄清华 吴婷[2] 杨阳[1,2] ZHANG Le-qi;LIN Cui-ting;HUANG Qing-hua;WU Ting;YANG Yang(Affiliated Foshan Maternity&Child Healthcare Hospital,Southern Medical University,Foshan 528000,Guangdong,China;School of Pharmaceutical Sciences,Southern Medical University,Guangzhou 510515,Guangdong,China)

机构地区：[1]南方医科大学附属佛山市妇幼保健院,中国广东佛山528000 [2]南方医科大学药学院,中国广东广州510515

出　　处：《生命科学研究》2022年第2期125-130,共6页Life Science Research

基　　金：国家自然科学基金-青年科学基金项目(82104256);广东省基础与应用基础研究基金区域联合基金-青年基金项目(2020A151511-0876);2020年南方医科大学大学生创新训练项目(X202012121344)。

摘　　要：高尿酸血症(hyperuricemia,HUA)在人类中具有较高的发病率,且其与痛风性关节炎、Ⅱ型糖尿病、心血管疾病以及肝肾功能损伤等多种疾病的进程密切相关。实验动物研究显示,HUA相关并发症需要长期稳定的诱导过程。与人类不同的是,常用模式动物具有较高的尿酸酶表达,可迅速将尿酸分解成可溶性尿囊素排泄出体外,该特点易造成造模期间高尿酸日均暴露时间不足。因此,HUA相关并发症的观察对实验模型在模式动物、诱导方式、高尿酸暴露时间等方面均有较高的要求。本文对HUA动物模型造模方法以及不同造模方法对慢性并发症的影响进行综述,重点从主流药物造模方法的优劣分析、模式动物的选择、基因干预造模3个方面展开,为HUA长期并发症研究的造模方法选择提供参考依据。Hyperuricemia(HUA)is a common disease associated with the incidence and development of multiple complications such as gouty arthritis,type 2 diabetes,cardiovascular disease,functional liver and kidney injuries.Long-term and stable induction is essential for HUA-associated complications in modeling animals.Unlike in human,common model animals express high levels of uricase that decompose uric acid to soluble allantoin for renal excretion.This profile leads to insufficient daily exposure to high uric acid levels during modeling period.Therefore,the model animal selection,induction method and high uric acid duration are all causes that influence the observation of hyperuricemia-associated complications.Herein,the research progress in modeling of long-term chronic HUA animals and evaluation of the associated complications is reviewed.It mainly focuses on evaluation of current chemical inducers,selection of modeling animals and influence of genetic intervention,in order to provide information for modeling method selection in long-term chronic HUA research.

关 键 词：高尿酸血症(HUA) 造模 并发症 

分 类 号：Q95-336[生物学—动物学] R589.7[医药卫生—内分泌]