基因枪介导BYDV-GPV-CP和Bar基因共转化小麦幼胚愈伤组织  

Gene Gun Mediated BYDV GPV-CP and Bar Genetic Transformation to Wheat Immature Embryo Callus

作　　者：崔志钢[1] 王琨[1] 赵会芳[1] 陈耀峰[2] CUI Zhi-gang;WANG Kun;ZHAO Hui-fang;CHEN Yao-feng(College of Agronomy,Tongren Polytechnic College,Tongren 554300,China;College of Agronomy,Northwest Agriculture & Forestry University,Yangling 712100,China)

机构地区：[1]铜仁职业技术学院农学院,贵州铜仁554300 [2]西北农林科技大学农学院,陕西杨凌712100

出　　处：《黑龙江农业科学》2022年第5期18-21,共4页Heilongjiang Agricultural Sciences

基　　金：铜仁市六大主导产业中园林元素与休闲农业融合发展创新团队(铜职院办发〔2020〕118号)。

摘　　要：为了优化小麦基因枪转化技术体系,提高小麦基因枪转化效率,分别以小麦品种小偃22、千斤早、西农979、扬麦18和扬麦12诱导9 d的幼胚愈伤组织为受体材料,使用基因枪介导BYDV-GPV-CP基因和抗除草剂Bar基因进行小麦幼胚愈伤组织共转化。结果表明:5种基因型小麦幼胚材料愈伤组织诱导效果存在显著差异,在接受基因枪轰击后再生苗率存在显著差异。在基质中添加4 mg·L^(-1)多效唑后再生苗平均分蘖最多,数量达6.47,证实在基质中添加4.0 mg·L^(-1)多效唑,可以有效促进壮苗和增加分蘖。In order to optimize wheat genetic transformation system and improve the efficiency of genetic transformation by gene gun,we used immature embryo callus as receptor materials which induced nine days come from Xiaoyan 22,Qianjinzao,Xinong 979,Yangmai 18,Yangmai 12 as materials,and used gene gun to co-transform BYDV-GPV-CP gene and herbicide resistance Bar gene into wheat immature embryo callus.Experiments confirmed that,under the same conditions,immature embryo callus induction rate of five different genotypes is significant difference.Confrontational plants had the most tillering with 4 mg·L^(-1) Paclobutrazol,the number reached 6.47.The results showed that adding 4.0 mg·L^(-1) paclobutrazol to the medium could effectively promote seedling growth and increase tillering.

关 键 词：基因枪 愈伤组织 基因型 再生苗 

分 类 号：S51[农业科学—作物学]