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作 者:储博彦 赵玉芬 郭淑英[2] 马书燕[2] CHU Bo-yan;ZHAO Yu-fen;GUO Shu-ying;MA Shu-yan(Hebei Academy of Forestry and Grassland Science,Shijiazhuang 050061,China;Department of Agriculture and Forestry Engineering,Tangshan Vocational and Technical College,Tangshan 063000,China)
机构地区:[1]河北省林业和草原科学研究院,河北石家庄050061 [2]唐山职业技术学院农林工程系,河北唐山063000
出 处:《黑龙江农业科学》2022年第5期64-68,共5页Heilongjiang Agricultural Sciences
基 金:河北省科技厅重点研发计划项目(19226359D)。
摘 要:为促进丝棉木化学诱变技术研究,以丝棉木成熟种子及幼苗作为试材,以蒸馏水浸泡种子作为对照,采用0%、0.05%、0.10%、0.15%和0.20%浓度梯度的秋水仙素分别处理12,24,36和48 h,测定分析秋水仙素处理后丝棉木种子发芽、胚根生长指标及幼苗苗高、地径生长状况。结果表明:随着秋水仙素处理浓度的增加和处理时间的增长丝棉木种子的发芽率和出苗率显著降低,部分种子幼胚变短变粗,胚根生长量降低,幼苗生长受到抑制。秋水仙素低浓度(0.10%以下)处理12 h有利于丝棉木种子的萌发。In order to promote the research of chemical mutagenesis technology of Euonymus bungeanus,the mature seeds and seedlings of Euonymus bungeanus were used as test materials,and the seeds soaked in distilled water were used as control.Colchicine with five different concentration gradients of 0%,0.05%,0.10%,0.15%and 0.20%were treated for 12,24,36 and 48 hours respectively.The seed germination,radicle growth index,seedling height and ground diameter growth of seedlings after colchicine treatment were measured and analyzed.The results showed that with the increase of colchicine treatment concentration and treatment time,the germination rate and seedling emergence rate of Euonymus bungeanus seeds decreased significantly,the young embryos of some seeds became shorter and thicker,the growth of radicle decreased,and the growth of seedlings was inhibited.The seeds treated with colchicine at low concentration(below 0.10%)for 12 h were conducive to seed germination.
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