吊石苣苔叶片愈伤组织诱导过程中生理生化指标变化  

Change of Physiological and Biochemical Indexes During Callus Induction of Lysionotus pauiflorus Leaves

作　　者：窦全丽[1] 杨秋婷 张仁波[1] 周远平 唐小迪 DOU Quan-li;YANG Qiu-ting;ZHANG Ren-bo;ZHOU Yuan-ping;TANG Xiao-di(Department of Biology and Agricultural Science and Technology,Zunyi Normal College,Zunyi 563000,China;Lancang County Third Nationality Middle School,Puer 665000,China)

机构地区：[1]遵义师范学院生物与农业科技学院,贵州遵义563000 [2]澜沧县第三民族中学,云南普洱665000

出　　处：《黑龙江农业科学》2022年第5期91-96,共6页Heilongjiang Agricultural Sciences

基　　金：遵义市科技局项目(遵市科合计〔2018〕11号);遵义师范学院博士基金(遵师BS〔2018〕17号)。

摘　　要：为提高吊石苣苔叶片组织培养效率,以同一生长期的吊石苣苔叶片为外植体进行组织培养,测定培养材料中的总蛋白质、超氧化物歧化酶(SOD)、过氧化物酶(POD)和丙二醛(MDA)等指标,分析吊石苣苔叶片愈伤组织诱导过程中的生理生化指标变化情况。结果表明:随着叶片的发育,SOD和POD活性总体呈先升后降再升的变化趋势,均在14 d达到峰值。MDA含量在培养14~21 d总体上升,到培养28 d由于POD和SOD含量的升高,清除细胞内的活性氧,使MDA含量降低。总蛋白质含量前14 d无明显变化,培养21 d含量达到峰值,之后再降低,整体呈先升后降的趋势。可溶性糖含量培养后呈先升后降再升的趋势,培养28 d达到峰值。In order to improve the efficiency of leaf tissue culture of Lysionotus pauiflorus,the leaves of Lysionotus pauiflorus at the same growth status were used as explants for tissue culture.Physiological and biochemical indexes,such as total protein,superoxide dismutase (SOD),peroxidase (POD),and malondialdehyde (MDA) were determined,and the changes of physiological and biochemical indexes were analyzed in the callus induction process.The results showed that the activities of SOD and POD increased first,then decreased and then increased,and reached the peak at 14 d of culture.After callus induction,MDA content generally increased during 14-21 d of culture.At 28 d of culture,MDA content decreased by scavenging reactive oxygen in cellsdue to the increase of POD and SOD activities.There was no significant difference in total protein content during 1-14 d while the content gradually increased to the peak and then decreased at 21 d of culture.The soluble sugar content increased first,then decreased and then increased after culture,and reached the peak at 28 d of culture.

关 键 词：吊石苣苔 叶片 愈伤组织诱导 生理生化指标 

分 类 号：R28[医药卫生—中药学]