甘薯近缘野生资源的染色体倍性研究  

Research on Chromosome Number of A Sweet Potato Wild Species Ipomoea trifida

作　　者：吉士东[1] 王丽[1] 胡启国 杨爱梅 JI Shidong;WANG Li;HU Qiguo;YANG Aimei(College of Life Science and College of Agricultural Engineering,Nanyang Normal University,Nanyang He’nan 473061,China;Shangqiu Academy of Agriculture and Forestry Sciences,Shangqiu He’nan 476000,China)

机构地区：[1]南阳师范学院生命科学与农业工程学院,河南南阳473061 [2]商丘市农林科学院,河南商丘476000

出　　处：《种子》2022年第4期121-125,共5页Seed

基　　金：河南省高等学校重点科研项目(16 B 180002);南阳师范学院博士专项(70351、15076);河南省高等学校大学生创新训练计划项目(202010481033)。

摘　　要：以甘薯近缘野生种Ipomoea trifida根尖为材料,分析0.1%秋水仙素、对二氯苯饱和溶液、2 mmol/L的8-羟基喹啉三种试剂不同处理时间对细胞有丝分裂指数、制片效果的影响。结果表明,对二氯苯饱和溶液对甘薯野生种I.trifida根尖进行3 h处理,细胞有丝分裂指数最高,染色体制片效果较好,为最佳预处理方法。基于上述体系鉴定了本试验中的甘薯野生种I.trifida为二倍体(2 n=2 x=30),秋水仙素加倍处理后的I.trifid是四倍体(2 n=4 x=60),徐薯18是六倍体(2 n=6 x=90)。Using the root tips of the closely related wild species Ipomoea trifida as materials.The three reagents including 0.1%colchicine,p-dichlorobenzene saturated solution and 2 mmol/L 8-hydroxyquinoline were used to analyze the effects on slicing of root tip in different treatment time,the root tips of Ipomoea trifida from a wild relative species of sweet potato.The highest mitotic index and the optimal slide specimen were obtained when root tips were treated with p-dichlorobenzene saturated solution for 3 hours.Based on the above system,the wild species of sweet potato I.trifida was idendified as diploid,I.trifida identified as tetraploid after colchicine doubling,Xinshu 18 identified as hexaploid.

关 键 词：甘薯野生种 染色体数 秋水仙素 对二氯苯 8-羟基喹啉 

分 类 号：S531[农业科学—作物学]