MTHFR、PAI-1基因多态性与反复性流产的关系研究  

Study on the correlation between MTHFR,PAI-1 gene polymorphisms and recurrent miscarriage

作　　者：张年宝[1] 赵梦岚 ZHANG Nianbao;ZHAO Menglan(Department of Pharmacy,People’s Hospital of Xuancheng City,Anhui,Xuancheng 242099,China)

机构地区：[1]安徽省宣城市人民医院药学部,242099

出　　处：《河北医药》2022年第9期1338-1341,共4页Hebei Medical Journal

基　　金：中华医学会临床药学分会-吴阶平医学基金会科研专项基金(编号:320.6750.19090-18)。

摘　　要：目的观察亚甲基四氢叶酸还原酶(MTHFR)、纤溶酶原激活剂抑制物-1(PAI-1)多态性与反复性流产的关系。方法选取2018年1月至2020年1月接受检查的338例孕妇,检测MTHFR基因C677T及1298基因型、PAI-1基因4G/5G基因型频率分布,记录孕妇既往流产情况,孕妇分为反复性流产组108例和非反复性流产组230例,比较2组基线资料及基因型频率分布,Logistic回归分析检验基因多态性与反复性流产的关系。结果2组年龄、孕前体重指数(BMI)、停经时间、孕次、孕期每天睡眠时间、孕期有无性生活比较,差异无统计学意义(P>0.05);2组MTHFR基因C677T基因型频率及PAI-1基因型频率分布比较,差异有统计学意义(P<0.05);2组MTHFR基因A1298C基因型频率分布比较,差异无统计学意义(P>0.05);反复性流产组总突变T等位基因频率高于非反复性流产组,4G等位基因频率高于非反复流产组,差异有统计学意义(P<0.05);Logistic回归分析显示,MTHFR基因C677T基因型及PAI-1基因4G/5G基因型频率、等位基因频率与反复性流产的发生密切相关,基因型TT、CT、4G4G,C677T等位基因T,4G/5G等位基因4G是反复性流产发生的风险因子(OR>1,P<0.05)。结论MTHFR、PAI-1基因多态性与反复性流产的发生密切相关。Objective To investigate the correlation between methylenetetrahydrofolate reductase(MTHFR),plasminogen activator inhibitor-1(PAI-1)polymorphisms and recurrent miscarriage.Methods A total of 338 pregnant women who received examination in our hospital from January 2018 to January 2020 were enrolled in the study.The frequency distributions of MTHFR gene C677T and 1298 genotypes,PAI-1 gene 4G/5G genotypes were detected,and previous abortion status of pregnant women was recorded.The pregnant women were divided into recurrent miscarriage group(n=108)and non recurrent miscarriage group(n=230),and baseline data and genotype frequency distributions were observed and compared between the two groups,Logistic regression analysis was performed to analyze the correlation between gene polymorphism and recurrent miscarriage.Results There were no significant differences in age,body mass index(BMI)before pregnancy,menopause time,pregnancy times,daily sleep time and asexual life during pregnancy between the two groups(P>0.05).There were significant differences in the frequency distributions of MTHFR gene C677T genotypes and PAI-1 genotypes between the two groups(P<0.05),however,there was no significant difference in the frequency distribution of MTHFR gene A1298C genotype between the two groups(P>0.05).The total mutation T allele frequency in recurrent miscarriage group was significantly higher than that in non recurrent miscarriage group,however,the 4G allele frequency was significantly higher than that in non recurrent miscarriage group(P<0.05).Logistic regression analysis showed that the frequencies of MTHFR gene C677T genotypes and PAI-1 gene 4G/5G genotype and allele were closely related to recurrent miscarriage,moreover,the genotypes TT,CT,4G4G,C677T allele T,4G/5G allele 4G were risk factors of recurrent miscarriage(P<0.05).Conclusion THFR and PAI-1 gene polymorphisms are closely related to the occurrence of recurrent miscarriage.

关 键 词：亚甲基四氢叶酸还原酶 纤溶酶原激活剂抑制物-1 基因多态性 反复性流产 

分 类 号：R714.21[医药卫生—妇产科学]