染色体核型、性激素、免疫性抗体检测在女性生殖异常患者中的应用  

Application of chromosome karyotype analysis,and detection of sex hormone and immune antibodies in female patients with reproductive abnormalities

作　　者：郝润英[1] 李佳[1] 袁茜 平慧[1] HAO Runying;LI Jia;YUAN Qian(Department of Clinical Laboratory,Handan Central Hospital,Hebei,Handan 056000,China)

机构地区：[1]河北省邯郸市中心医院检验科,056000

出　　处：《河北医药》2022年第9期1354-1356,1360,共4页Hebei Medical Journal

摘　　要：目的探讨染色体核型分析、性激素、免疫性抗体检测在女性生殖异常患者中的应用价值。方法选取2015年7月至2019年12月就诊的女性生殖异常患者423例作为观察组,另选取同期进行健康体检的正常女性150例作为对照组。对比2组外周血染色体核型、性激素[卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)、孕酮(P)]、免疫性抗体[抗子宫内膜抗体(EMAb)、抗精子抗体(AsAb)、抗卵巢抗体(AoAb)]的差异。结果对照组伴有染色体异常1例,检出率为0.67%(1/150),观察组伴有染色体异常16例,检出率为3.78%(16/423),观察组染色体异常检出率显著高于对照组(P<0.05)。观察组16例染色体异常患者中,包括染色体结构异常9例,其中相互易位4例,罗氏易位2例,倒位3例;染色体数量异常及嵌合体6例,性反转1例。观察组FSH、LH、P水平及EMAb、AsAb、AoAb阳性率显著高于对照组,E2水平显著低于对照组,差异均有统计学意义(P<0.05),且生殖异常患者中染色体异常者FSH、LH、P水平及EMAb、AsAb、AoAb阳性率显著高于染色体正常者,E2水平显著低于染色体正常者,差异均有统计学意义(P<0.05)。结论女性生殖异常患者多伴有染色体核型、性激素及免疫性抗体异常,是造成女性不孕和不良孕产的重要原因,联合检测对于女性生殖异常患者临床诊断和治疗具有重要价值。Objective To investigate the application value of chromosome karyotype analysis,and detection of sex hormone and immune antibodies in female patients with reproductive abnormalities.Methods A total of 423 female patients with reproductive abnormalities who were treated in our hospital from July 2015 to December 2019 were enrolled as observation group,at the same tiem,the other 150 healthy women who underwent physical examination were enrolled as control group.The chromosome karyotype in peripheral blood,and the serum levels of sex hormones(FSH,LH,E2 and P)and immune antibodies(EMAb,AsAb and AoAb)were observed and compared between the two groups.Results The detection rate of abnormal karyotype in observation group was 3.78%(16/423),which was significantly higher than that[0.67%(1/150)]in control group(P<0.05).Among the 16 patients with chromosome abnormality in observation group,there were 9 case of chromosomal structural abnormality(4 cases of reciprocal translocation,2 cases of Robertsonian translocation,and 3 cases of inversion),there were 6 case of chromosomal number abnormality,and 1 case of sex reversal syndrome.The serum levels of FSH,LH,P and the positive rates of EMAb,AsAb,AoAb in observation group were significantly higher than those in control group,however,the E2 levels were significantly lower than those in control group(P<0.05).Moreover the serum levels of FSH,LH,P and the positive rates of EMAb,AsAb,AoAb in the patients with chromosome abnormalities group were significantly higher than those of patients with normal chromosome,but the E2 levels were significantly lower than those of patients with normal chromosome(P<0.05).Conclusion The female patients with reproductive abnormalities are often associated with the abnormalities of chromosome karyotype,sex hormone and immune antibodies,which are important causes resulting in female sterility and bad pregnancy.Therefore,it is of great clinical significance to strengthen the combination detection of chromosome,sex hormone and immune antibodies for the di

关 键 词：生殖异常 染色体核型 性激素 免疫性抗体 

分 类 号：R339.22[医药卫生—人体生理学]