高桥仁蚧线粒体全基因组测序与分析  

Sequencing and analysis of the complete mitochondrial genome of Aclerda takahashii(Hemiptera:Aclerdidae)

作　　者：邓鋆 王刚 卢聪聪 张江涛 黄晓磊 DENG Jun;WANG Gang;LU Cong-Cong;ZHANG Jiang-Tao;HUANG Xiao-Lei(State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China;Key Laboratory of State Forestry and Grassland Administration on Forest Ecosystem Protection and Restoration in Poyang Lake Watershed, College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China)

机构地区：[1]福建农林大学植物保护学院,闽台作物有害生物生态防控国家重点实验室,福州350002 [2]江西农业大学林学院,鄱阳湖流域森林生态系统保护与修复国家林业和草原局重点实验室,南昌330045

出　　处：《昆虫学报》2022年第4期451-459,共9页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(31970446);国家重点研发计划项目(2016YFE0203100)。

摘　　要：【目的】测定和分析半翅目(Hemiptera)仁蚧科(Aclerdidae)首个线粒体全基因组——高桥仁蚧Aclerda takahashii线粒体全基因组序列,并探讨与其他蚧虫类群的系统发育关系。【方法】基于Illumina测序技术进行高桥仁蚧线粒体全基因组测序,并进行生物信息学分析;基于已报道的15科31种半翅目昆虫的线粒体全基因组序列运用最大似然法(ML)和贝叶斯推断法(BI)构建半翅目系统发育树。【结果】高桥仁蚧线粒体基因组全长16599 bp,AT含量高达84.51%。在该线粒体基因组中,缩减tRNA非常普遍,10个tRNA缺失二氢尿嘧啶(DHU)臂或TΨC臂,tRNA^(ser(S1))和tRNA^(ser(S2))缺失DHU臂和TΨC臂。系统发育树显示,与仁蚧科亲缘关系最近的是蚧科(Coccidae)。【结论】本研究报道了首个仁蚧科的线粒体基因组,发现在高桥仁蚧线粒体基因组中存在普遍的tRNA缺臂现象,为进一步系统地研究蚧虫线粒体基因组提供了数据支持。【Aim】This study aims to sequence and analyze the complete mitochondrial genome(mitogenome)of Aclerda takahashii,which represents the first complete mitogenome of the family Aclerdidae of Hemiptera,and to investigate the phylogenetic relationships with other Coccoidea groups.【Methods】The whole mitogenome of A.takahashii was sequenced based on the Illumina sequencing technique,and analyzed with bioinformatics methods.The phylogenetic trees of hemipteran insects were constructed based on the reported complete mitochondrial genomes of 31 speciec of 15 families of Hemiptera using the maximum likelihood(ML)and Bayesian inference(BI)methods.【Results】The mitogenome of A.takahashii is 16599 bp in length with high A+T content(84.51%).The truncated tRNAs are common in this mitogenome,ten tRNAs lacking of a dihydrouridine(DHU)arm or TΨC(T)arm.The DHU and T arm of tRNA^(ser(S1))and tRNA^(ser(S2))are both missing.The phylogenic trees showed that Aclerdidae has the closest relationship with Coccidae.【Conclusion】This study reported the first mitogenome of Aclerdidae,and revealed that truncated tRNAs are prevalent in the mitogenome of A.takahashii,providing data for the further systematic research of mitogenomes of scale insects.

关 键 词：半翅目 仁蚧科 高桥仁蚧 线粒体基因组 缩减tRNAs 系统发育 

分 类 号：Q969.35[生物学—昆虫学]