林麝FASN基因CDS区克隆及序列分析  

Cloning and Sequence Analysis of FASN Gene in the CDS Region of Moschus berezovskii

作　　者：谌颖莲 竭航[1] 赵贵军[1] 封孝兰[1] 张承露[1] 吴佳勇 曾德军[1] CHEN Yinglian;JIE Hang;ZHAO Guijun;FENG Xiaolan;ZHANG Chenglu;WU Jiayong;ZENG Dejun(Key Laboratory of Sichuan and Chongqing Co-construction for Characteristic Biological Resources Research and Utilization,Chongqing Institute of Drug Cultivation,Chongqing,408435,China)

机构地区：[1]重庆市药物种植研究所,特色生物资源研究与利用川渝共建重点实验室,重庆408435

出　　处：《野生动物学报》2022年第2期370-378,共9页CHINESE JOURNAL OF WILDLIFE

基　　金：国家自然科学基金项目(81973428);重庆市技术创新与应用发展专项项目(cstc2019jscx-dxwtBX0021);重庆市自然科学基金项目(cstc2019jcyj-msxmX0024)。

摘　　要：为了解林麝(Moschus berezovskii)脂肪酸合酶(Fatty acid synthase,FASN)基因mRNA序列信息及解析麝香的生物机制,通过转录组测序、Trinity拼接、反转录PCR扩增和Singer测序获取林麝FASN基因mRNA序列,并对FASN蛋白序列进化树构建、二级结构、三级结构、互作蛋白、磷酸化分析及miRNAs结合预测,筛选与麝鼠(Ondatra zibethicus)FASN相同且与牛(Bos taurus)、山羊(Capra hircus)和绵羊(Ovis aries)不同的氨基酸位点作为麝香生物合成有关的关键位点。获取了林麝FASN基因的mRNA序列(GenBank ID:MW570770),其具有一定的物种保守性,筛选出16个关键位点,其中第307、602、657和1334位氨基酸能够改变蛋白羟基活性,预测出10个蛋白磷酸化位点,miR-1307-5p和miR-669与3′-UTR亲和能力较强。林麝FASN基因序列与其他物种之间的差异信息可为解析麝香生物合成机制提供参考。In order to better understand mRNA sequence information of FASN gene and the musk biosynthesis mechanism of Moschus berezovskii,the mRNA sequence of FASN gene had been acquired by the transcriptomic sequencing,Trinity splicing,reverse transcription PCR amplification and Singer sequence.Then,the construction of evolutionary tree,prediction of secondary and tertiary structures,analysis of protein interactions,phosphorylation site as well as miRNA affinity predictions had been performed and shown in this paper.The same amino acid sites which are consistent with muskrat(Ondatra zibethicus)but different with the cattle(Bos taurus),goat(Capra hircus)and sheep(Ovis aries)in the FASN,had been screened and considered as the critical sites for musk biosynthesis.The mRNA sequence(GenBank ID:MW570770)of FASN gene of Moschus berezovskii had been acquired by transcriptomic sequencing and clone sequence and displayed certain species conserved.There were 16 amino acid sites related to musk synthesis where the 307th,602th,657th and 1334th sites were referred to change the activity of hydroxyl functional groups.Ten phosphorylation sites and two miRNAs including miR-1307-5p and miR-669 strongly binding 3′-UTR had been predicted.The differential information between M.berezovskii and other species on FASN gene sequence could be provided and considered as reference for the study of musk biosynthesis mechanism.

关 键 词：林麝 FASN 蛋白序列分析 麝香生物合成 MIRNAS 

分 类 号：Q953[生物学—动物学]