化学发光探针构建及应用进展  

Advances in the construction and application of chemiluminescence probes

作　　者：冯玉蓉 徐帅 宦双燕[1] 袁林 张晓兵[1] FENG Yurong;XU Shuai;HUAN Shuangyan;YUAN Lin;ZHANG Xiaobing(College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China)

机构地区：[1]湖南大学化学化工学院,湖南长沙410082

出　　处：《南通大学学报（自然科学版）》2022年第1期1-13,共13页Journal of Nantong University(Natural Science Edition)　

基　　金：国家自然科学基金重大项目(21890744);国家自然科学基金面上项目(21977027)。

摘　　要：化学发光过程中,当发光体系与响应底物发生化学反应后,体系中的染料分子被化学反应产生的化学能激发,随之产生光信号。该发光体系不需要外加激发光源,从而可以有效避免激发光源带来的背景荧光干扰,并显著提高成像对比度,使其在活体成像方面具有独特优势;此外,化学发光体系可以作为内在光源激发光敏剂用于肿瘤治疗。文章主要介绍当前化学发光体系的种类,包括鲁米诺、过氧草酸酯、1,2-二氧杂环丁烷化学发光体系,以及其发光机理和结构特征,并着重总结了其在生物成像、治疗中的应用。最后,对化学发光体系的发展方向进行了展望,指出发展新型结构的长波长、高亮度的化学发光体系是提高生物成像和治疗效果的有效策略。In the chemiluminescence process, when the luminescent system and the response substrate undergo a chemical reaction, the dye molecules in the system are excited by the chemical energy generated by the chemical reaction and generate light signals. Since the external excitation light source is not required, the interference of background fluorescence is avoided and the imaging contrast will be improved, making it have unique advantages in in vivo imaging.In addition, the chemiluminescence system can be used as an internal light source to excite the photosensitizer for tumor therapy. In this review, the current type of luminescence systems, including Luminol, peroxyoxalate, 1, 2-dioxetane chemiluminescence system, as well as their luminescence mechanism and structural characteristics are reviewed,and their application in imaging and therapy is summarized. Finally, the development direction of these systems is prospected, and it is revealed that the development of a long-wavelength, high-brightness chemiluminescence system with a new structure is an effective strategy to the improve bioimaging and therapeutic effects.

关 键 词：化学发光 鲁米诺 过氧草酸酯 1 2-二氧杂环丁烷 生物成像 肿瘤治疗 

分 类 号：O657.3[理学—分析化学] R730.5[理学—化学]