电针迷走神经对慢性不可预知性应激致幼鼠抑郁模型海马组织GFAP、NeuN表达的影响  

Effect of vagus nerve stimulation by electroacupuncture on GFAP and NeuN in hippocampal of juvenile rats with depression induced by chronic unpredictable stress

作　　者：宁秋香 彭红莉[1] 肖剑英[1] 杨超 周志强[1] 刘芳 梁小丽[1] 杨栋[1] Ning Qiuxiang;Peng Hongli;Xiao Jianying;Yang Chao;Zhou Zhiqiang;Liu Fang;Liang Xiaoli;Yang Dong(Department of Psychology,Brain Hospital of Hunan Province,Changsha 410007,China;Department of Pediatric Psychiatry,Urumqi Fourth People′s Hospital,Urumchi 830002,China)

机构地区：[1]湖南省脑科医院精神科,长沙410007 [2]乌鲁木齐第四人民医院儿童心理科,乌鲁木齐830002

出　　处：《中国医师杂志》2022年第4期538-542,共5页Journal of Chinese Physician

基　　金：湖南省重点研发项目(2020SK2123);新疆维吾尔自治区自然科学基金(2019D01A13);湖南省卫生计生委项目(202103090662,202203094023);湖南省脑科医院院级课题(2018D07,2017D01)。

摘　　要：目的分析基于慢性不可预知性应激(CUS)致幼鼠抑郁模型海马组织胶质纤维酸性蛋白(GFAP)、神经元核心(NeuN)抗原表达的情况,探讨电针迷走神经对CUS抑郁模型的影响。方法选取SD幼鼠6只作为对照组(不给予任何刺激),其余进行CUS造模后采用随机数字法分为CUS组、伪刺激组、氟西汀组及电针组,每组各6只。氟西汀组予以10 mg/kg氟西汀干预,对照组、CUS组予以等量生理盐水干预;电针组结扎迷走神经后刺激迷走神经远端,伪刺激组仅分离迷走神经但不予电刺激。干预28 d后五组进行旷场实验及糖水实验,HE染色观察幼鼠海马神经元细胞,免疫组化检测幼鼠海马GFAP、NeuN表达情况。结果CUS造模后干预前,CUS组、伪刺激组、氟西汀组、电针组垂直运动次数、水平运动次数及糖水消耗、糖水偏爱的程度均明显低于对照组,差异有统计学意义(均P<0.01);干预后,CUS组、伪刺激组以上指标仍低于对照组(均P<0.01),但氟西汀组、电针组上述指标明显高于CUS组、伪刺激组(均P<0.01)。HE染色显示,CUS组、伪刺激组海马神经元细胞排列疏松,并出现细胞肿胀及固缩,氟西汀组、电针组则明显改善。免疫组化结果显示,与对照组相比,CUS组、伪刺激组幼鼠海马组织GFAP表达增加、NeuN表达下降(均P<0.01);与CUS组、伪刺激组比较,氟西汀组、电针组GFAP表达下降、NeuN表达增加(均P<0.01)。结论电针迷走神经可明显改善幼鼠抑郁症状,与氟西汀作用相当,可能与调整海马区GFAP、NeuN表达有关。Objective To analyze the expression of glial fibrillary acidic protein(GFAP)and neuronal nuclei(NeuN)antigen in hippocampus based on the depression model of juvenile rats caused by chronic unpredictable stress(CUS),and to explore the effect of electroacupuncture vagus nerve on CUS depression model.Methods Six juvenile SD rats were selected as the control group(without any stimulation),and the rest were divided into CUS group,pseudo stimulation group,fluoxetine group and electroacupuncture group by random number method after CUS modeling,with 6 rats in each group.Fluoxetine group was given 10 mg/kg fluoxetine intervention;control group and CUS group were given the same amount of normal saline intervention;In the electroacupuncture group,the distal vagus nerve was stimulated after ligation,while in the pseudo stimulation group,only vagus nerve was isolated without electrical stimulation.After 28 d of intervention,the five groups were subjected to Open-field Test and Sucrose Preference Test.Hippocampal neurons were detected by hematoxylin and eosin(HE)staining,and the expressions of GFAP and NeuN in hippocampal were detected by immunohistochemistry.Results After CUS modeling and before intervention,the number of vertical and horizontal movements,sucrose consumption and sucrose preference in CUS group,pseudo stimulation group,fluoxetine group and electroacupuncture group were significantly lower than those in the control group(all P<0.01);After the intervention,the above indexes in CUS group and pseudo stimulation group were still lower than those in the control group(all P<0.01),but the above indexes in fluoxetine group and electroacupuncture group were significantly higher than those in CUS group and pseudo stimulation group(all P<0.01).HE staining showed that the arrangement of hippocampal neurons in CUS group and pseudo stimulation group were loose,and there were cell swelling and pyknosis,which was significantly improved in fluoxetine group and electroacupuncture group.Immunohistochemical results showed that com

关 键 词：迷走神经刺激术 疾病模型 动物 抑郁 应激 心理学 神经胶质原纤维酸性蛋白质 

分 类 号：R245.97[医药卫生—针灸推拿学]