石墨烯的氧化与抗菌特性  

Oxidation and Antibacterial Properties of Graphene

作　　者：周可心 苗泽萌 杨勇 黄小露 张耀中 李新红 张亚非 Zhou Kexin;Miao Zemeng;Yang Yong;Huang Xiaolu;Zhang Yaozhong;Li Xinhong;Zhang Yafei(Key Laboratory of Thin Film and Microtechnology of Ministry of Education,School of Electronic Information and Electrical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学电子信息与电气工程学院薄膜与微细技术教育部重点实验室,上海200240

出　　处：《微纳电子技术》2022年第4期328-334,364,共8页Micronanoelectronic Technology

基　　金：上海市自然科学基金资助项目(17ZR1414100)。

摘　　要：氧化石墨烯(GO)的抗菌机制(破坏细菌结构、氧化应激和阻断细菌传输)只从宏观角度论述了GO整体结构对细菌的作用,但未能说明表面官能团对抗菌的贡献。在不同气氛(N_(2)或O_(2))下退火对石墨烯进行表面官能团修饰,得到表面官能团组成不同的氮气处理石墨烯(NTG)、氧气处理石墨烯(OTG)。平板菌落计数法抗菌测试表明,OTG有更加优异的抗菌性能,对大肠杆菌和金黄色葡萄球菌的抗菌率明显高于未处理的石墨烯和NTG的。为了进一步对OTG的结构和抗菌性能进行优化,得到最佳反应条件:环境温度200℃,反应气氛为O_(2),反应时间5h。最终OTG对大肠杆菌和金黄色葡萄球菌的抗菌率分别可达92.4%和93%。傅里叶变换红外光谱(FTIR)、热重分析法(TGA)、X射线光电子能谱(XPS)的结果表明,OTG的表面结构为碳氧键终止表面sp^(2)碳(定型碳),并得到表面碳氧键的形态和含量。结合抗菌测试结果,得出C=O键为抗菌主要贡献的结论。The existing antibacterial mechanisms of graphene oxide(GO)includes destruction of bacterial structure,oxidative stress and blocking of bacterial transmission,the effect of the overall structure of GO on bacteria is only discussed from a macro perspective,but the contribution of surface functional groups of graphene to antibacterial is not explained.The surface functional groups of graphene were modified by annealing at different atmospheres(N_(2)or O_(2))to obtain nitrogen-treated graphene(NTG)and oxygen-treated graphene(OTG)with surface functional groups at different compositions.Plate colony counting antibacterial test shows that OTG has better antibacterial performance,and antibacterial rates against Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)are significantly higher than those of untreated graphene and NTG.To further optimize the structure and antibacterial performance of OTG,the optimal reaction conditions are as follows:ambient temperature is 200℃,reaction atmosphere is O_(2)and reaction time is 5 h.Finally,the antibacterial rates of OTG against E.coli and S.aureus are 92.4%and 93%,respectively.The results of Fourier transform infrared spectroscopy(FTIR),thermo gravi-metry analysis(TGA)and X-ray photoelectron spectroscopy(XPS)show that OTG surface structure is the surface sp^(2) carbon(fixed carbon)terminated by the carbon oxygen bond,and the morphology and content of the surface carbon oxygen bond are obtained.Combined with the results of antibacterial test,it is concluded that C=O bond is the main contribution of antibacterial.

关 键 词：石墨烯 氧化石墨烯(GO) 碳纳米材料 抗菌性能 表面分析 

分 类 号：TB34[一般工业技术—材料科学与工程]