斜叶黄檀茎中1个新的肉桂酚类化合物  

A new cinnamylphenol from stems of Dalbergia pinnata

作　　者：谭少丽 易涛 杨伟群 江诗琴 张丹雁[1] 崔辉 赵钟祥[1] TAN Shao-li;YI Tao;YANG Wei-qun;JIANG Shi-qin;ZHANG Dan-yan;CUI Hui;ZHAO Zhong-xiang(School of Pharmaceutical Science,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Guangdong Key Laboratory of New Drug Design and Evaluation,School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,China)

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]中山大学药学院广东省新药设计与评价重点实验室,广东广州510006

出　　处：《中草药》2022年第8期2274-2277,共4页Chinese Traditional and Herbal Drugs

基　　金：广州中医药大学“双一流”与高水平大学学科协同创新团队重点项目(2021xk31);广东省新药设计与评价重点实验室开放课题基金(2017B030314030)。

摘　　要：目的研究斜叶黄檀Dalbergia pinnata茎部化学成分,并初步考察其抗炎活性。方法采用正相硅胶、Sephadex LH-20凝胶、ODS及制备型高效液相等柱色谱方法进行分离纯化,通过核磁共振波谱、质谱和红外光谱等技术进行结构鉴定;采用脂多糖(lipopolysaccharide,LPS)诱导RAW 264.7细胞一氧化氮生成模型对分离鉴定的化合物进行抗炎活性筛选。结果从斜叶黄檀茎的甲醇提取物中分离得到4个肉桂酚类化合物,分别鉴定为黄檀亭F(1)、2-羟基异尖叶军刀豆苏合香烯(2)、异尖叶军刀豆苏合香烯(3)和黄檀亭C(4)。结论化合物1为新化合物,化合物2~4为首次从斜叶黄檀中分离得到。化合物1对LPS诱导RAW 264.7细胞释放一氧化氮显示出抑制活性,半数抑制浓度为23.6μmol/L。Objective To study the chemical constituents and their anti-inflammatory activities from the stems of Dalbergia pinnata.Methods The constituents were separated and purified by various column chromatographic technologies(silica gel,Sephadex LH-20,ODS column chromatography)as well as HPLC.The structures were elucidated by MNR,MS and IR data.All compounds were evaluated for their inhibitory activity against LPS-activated NO production in RAW 264.7 cell model.Results Four compounds were isolated and identified as dalberatin F(1),2-hydroxyisomucronustyrene(2),isomucronustylene(3)and dalberatin C(4).Conclusion Compound 1 is identified as a new cinnamylphenol,and compounds 2—4 are isolated from this plant for the first time.Compound 1 exhibits inhibitory effect on NO production in LPS-induced RAW 264.7 cells,with the IC;value of 23.6μmol/L.

关 键 词：斜叶黄檀 肉桂酚 黄檀亭F 2-羟基异尖叶军刀豆苏合香烯 异尖叶军刀豆苏合香烯 黄檀亭C 抗炎活性 

分 类 号：R284.1[医药卫生—中药学]