忍冬叶化学成分及其保肝活性研究  

Chemical constituents from Lonicera japonica leaves and their hepatoprotective activities

作　　者：王凯 刘双 李蒙 李丽丽 于金倩 王晓 WANG Kai;LIU Shuang;LI Meng;LI Li-li;YU Jin-qian;WANG Xiao(School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250300,China;Shandong Key Laboratory for Applied Technology of Sophisticated Analytical Instruments,Shandong Analysis and Test Center,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China)

机构地区：[1]山东中医药大学药学院,山东济南250300 [2]齐鲁工业大学(山东省科学院)山东省分析测试中心山东省大型精密仪器应用技术重点实验室,山东济南250014

出　　处：《中草药》2022年第8期2285-2291,共7页Chinese Traditional and Herbal Drugs

基　　金：山东省重大科技创新工程(2021CXGC010508);国家自然科学基金项目(21904080);济南市“高校20条”项目(2020GXRC007)。

摘　　要：目的研究忍冬Lonicera japonica叶化学成分及其保肝活性。方法采用硅胶柱色谱、反相C_(18)柱色谱、Sephadex LH-20柱色谱、半制备型高效液相色谱等方法进行分离与纯化,并根据理化性质和波谱学数据分析鉴定化合物结构;利用体外肝细胞损伤模型,采用MTT法检测化合物单体抵抗对乙酰氨基酚(APAP)诱导的人肝癌HepG2细胞损伤的作用。结果从忍冬叶醇提物中分离得到19个化合物,分别鉴定为断氧化马钱苷酸(1)、8-表番木鳖酸(2)、马钱苷酸(3)、咖啡酸(4)、绿原酸(5)、隐绿原酸(6)、新绿原酸(7)、6′-O-β-D-呋喃芹糖基獐牙菜苷(8)、demethylsecologanol(9)、獐牙菜苷(10)、断马钱子苷半缩醛内酯(11)、表断马钱子苷半缩醛内酯(12)、木犀草素-7-O-β-D-葡萄糖苷(13)、异绿原酸A(14)、异绿原酸B(15)、木犀草苷(16)、木犀草素-7-O-[α-L-吡喃阿拉伯糖基-(1→6)]-β-D-吡喃葡萄糖苷(17)、木犀草素-7-O-木糖(18)、忍冬苷(19);其中化合物2~11、1~15、17具有较好的抗APAP肝损伤作用。结论化合物1、2、8、11、12、18均首次从忍冬叶中分离得到,其中化合物18首次从忍冬植物中分离得到,化合物2~11、13~15、17具有明显的保肝活性。Objective To study the chemical constituents of Lonicera japonica leaves and their hepatoprotective activities.Methods The chemical constituents from L.japonica leaves were isolated and purified by the chromatography on silica gel column,reversed-phase C_(18) column,Sephadex LH-20 column,and semi-preparative HPLC and their structures were identified by various spectroscopic analysis.In this experiment,an in vitro hepatocyte injury model was used,and the MTT method was used to detect the effects of compounds against acetaminophen(APAP)-induced injury of human liver cancer HepG2 cells.Results Nineteen compounds were isolated from the ethanol extract of L.japonica leaves,which were identified as secologanoside(1),8-epiloganic acid(2),loganic acid(3),caffeic acid(4),chlorogenic acid(5),cryptochlorogenic acid(6),neochlorogenic acid(7),6′-O-β-D-apiofuranosyl sweroside(8),demethylsecologanol(9),sweroside(10),vogeloside(11),epi-vogeloside(12),luteolin-7-O-β-D-glucoside(13),isochlorogenic acid A(14),isochlorogenic acid B(15),cynaroside(16),luteolin-7-O-[α-Lpyranafinosyl-(1→6)]-β-D-glucopyranoside(17),luteolin-7-O-xyloside(18)and lonicerin(19).Among them,compounds 2—11,13—15,17 showed significant anti-APAP liver injury effects.Conclusion Compounds 1,2,8,11,12 and 18 are isolated from L.japonica leaves for the first time,while compound 18 is obtained from L.japonica for the first time.Compounds 2—11,13—15,17 showed significant hepatoprotective activities.

关 键 词：忍冬叶 保肝活性 断氧化马钱苷酸 8-表番木鳖酸 6′-O-β-D-呋喃芹糖基獐牙菜苷 断马钱子苷半缩醛内酯 

分 类 号：R284.1[医药卫生—中药学]