小儿消积止咳口服液抗肠炎靶点与作用机制研究  

Anti-enteritis targets and mechanism of Xiaoer Xiaoji Zhike Oral Liquid

作　　者：罗倩微 姚璐 杨倬 姚景春 孙成宏 张贵民 曾克武[1] LUO Qian-wei;YAO Lu;YANG Zhuo;YAO Jing-chun;SUN Cheng-hong;ZHANG Gui-min;ZENG Ke-wu(State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University,Beijing 100191,China;State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine,Lunan Pharmaceutical Group Co.,Ltd.,Linyi 276006,China)

机构地区：[1]北京大学药学院天然药物及仿生药物国家重点实验室,北京100191 [2]鲁南制药集团股份有限公司中药制药共性技术国家重点实验室,山东临沂276006

出　　处：《中草药》2022年第8期2368-2375,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81973505);国家重点研发计划基金资助项目(2019YFC170892,2019YFC1711000)。

摘　　要：目的探索小儿消积止咳口服液改善肠道炎症作用的直接靶点,从炎症靶点生物学功能的角度,阐释小儿消积止咳口服液保护肠道并发挥消积功效的作用机制。方法基于靶点“钩钓”策略从脂多糖(lipopolysaccharide,LPS)诱导的小鼠肠道组织中捕获并通过高分辨质谱鉴定小儿消积止咳口服液提取物的直接作用靶点蛋白,基于京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)分析靶点蛋白相关信号通路。通过qRT-PCR、免疫组化等方法进行靶点蛋白的功能验证。结果累计从肠道组织鉴定到1333个结合蛋白,并通过KEGG分析发现靶点蛋白群主要与肠道动力学、肠道免疫、黏膜屏障等功能有关。进而利用免疫组化、qRT-PCR等方法确证了小儿消积止咳口服液具有上述保护肠道功能的潜在作用。结论小儿消积止咳口服液具有改善小鼠肠道组织炎症损伤的作用,其作用机制可能与增强肠道动力、改善肠道免疫微环境以及维持肠道黏膜完整性有关。Objective To explore the direct targets of Xiaoer Xiaoji Zhike Oral Liquid(小儿消积止咳口服液,XXZL)in improving intestinal inflammation,and explain the mechanism of XXZL on protecting the intestinal tract and exerting the effect of eliminating accumulation through the biological function of inflammatory targets.Methods The target proteins from intestinal tissues of lipopolysaccharide(LPS)-induced mice were captured by“target fishing”strategy,and identified by high resolution mass spectrometry,followed by signaling pathway analysis based on Kyoto encyclopedia of genes and genomes(KEGG).The functions of target proteins were verified by qRT-PCR and immunohistochemistry assays.Results A total of 1333 binding proteins were identified,and these target proteins were mainly related to intestinal dynamics,intestinal immunity and mucosal barrier.Moreover,the immunohistochemistry and qRT-PCR assays confirmed the potential effects of XXZL on intestinal protection.Conclusion XXZL can ameliorate LPS-induced intestinal tissue inflammation in mice,via enhancing intestinal motility,improving intestinal immune microenvironment and maintaining the integrity of intestinal mucosa.

关 键 词：小儿消积止咳口服液 肠炎 靶点鉴定 肠道动力 免疫微环境 

分 类 号：R285.5[医药卫生—中药学]