广地龙化学成分和药理作用的研究进展及其质量标志物(Q-Marker)的预测分析  

Research progress on chemical components and pharmacological effects of Pheretima aspergillum and predictive analysis on quality marker

作　　者：李思维 郝二伟 杜正彩[2,3,4] 韦玮 王星圆[1,2,3] 陈静梅 侯小涛[1,2,3] 邓家刚 LI Si-wei;HAO Er-wei;DU Zheng-cai;WEI Wei;WANG Xing-yuan;CHEN Jing-mei;HOU Xiao-tao;DENG Jia-gang(Faculty of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica,Nanning 530200,China;Guangxi Collaborative Innovation Center of Study on Functional Ingredients of Agricultural Residues,Nanning 530200,China;Guangxi Scientific Experimental Center of Traditional Chinese Medicine,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学药学院,广西南宁530200 [2]广西中药药效研究重点实验室,广西南宁530200 [3]广西农作物废弃物功能成分研究协同创新中心,广西南宁530200 [4]广西中医药大学广西中医药科学实验中心,广西南宁530200

出　　处：《中草药》2022年第8期2560-2571,共12页Chinese Traditional and Herbal Drugs

基　　金：中国(广西)-东盟药物创新中心建设及新药开发项目(桂科AA19254033);2020年广西中药药效研究重点实验室(20-065-38)。

摘　　要：广地龙Pheretima aspergillum为我国常用传统中药材,主要分布于广西、广东,为岭南地区道地药材。广地龙中化学成分包括蛋白质及多肽类、酶类、氨基酸及二肽类、核苷类、脂肪酸类、甾体化合物、溶血磷脂、无机元素等。传统认为广地龙蚓激酶、次黄嘌呤和琥珀酸为其主要药效成分。对广地龙化学成分、药理作用的研究进展进行总结,并在此基础上,分析酶类、核苷类、脂肪酸类等成分与药效的关联,基于传统功效与现代药理作用预测其质量标志物(quality marker,Q-Marker),初步确定纤溶活性蛋白、蚓激酶、蚯蚓纤溶酶、次黄嘌呤、肌苷、琥珀酸、多不饱和脂肪酸、氨基酸及二肽类成分为其可能的Q-Marker。建议聚焦其有效成分,结合成分化学物质组入血后的协同作用以及化学成分可测性,建立专属性的测定方法,为明确广地龙Q-Marker和制定科学的质量评价体系提供基础。Pheretima aspergillum,a commonly used traditional Chinese medicine is mainly distributed in Lingnan areas of China,and Guangdong and Guangxi Provinces are its genuine producing areas.The chemical composition of P.aspergillum are proteins and peptides,enzymes,amino acids and dipeptides,nucleosides,fatty acid,steroids,lysoglycerophospholipids and inorganic elements,etc.Traditionally,lumbrokinase,hypoxanthine and succinic acid were considered as the main effective components of P.aspergillum.In this paper,the chemical composition and pharmacological effects of P.aspergillum were summarized.Based on above,the correlation between enzymes,nucleotides,fatty acid and pharmacodynamics was analyzed,and the quality marker(Q-Marker)of P.aspergillum were predicted based on traditional efficacy and modern pharmacology.As a result,the fibrinolytic protein,lumbrokinase,earthworm fibrinolytic enzyme,hypoxanthine,inosine,succinic acid,polyunsaturated fatty acid,amino acids and dipeptides were preliminarily determined as the possible Q-Markers.It is suggested that specific determination methods should be established by focusing on the active constituents,combining with synergistic effect of chemical substances in blood and measurability of chemical substances,so as to provide basis for Q-Marker determination and quality assessment.

关 键 词：广地龙 药效物质基础 质量标准 质量标志物 纤溶活性蛋白 蚓激酶 蚯蚓纤溶酶 次黄嘌呤 肌苷 琥珀酸 多不饱和脂肪酸 氨基酸及二肽类 

分 类 号：R282.710.5[医药卫生—中药学]