基于介孔纳米碳球接枝聚胍类化合物协同光热抗菌策略的研究  

Research of Synergistic Photothermal Antibacterial Strategy Based on Polymeric Guanidine Derivative Grafted on Mesoporous Carbon Nanospheres

作　　者：张审 冯闪 马陇豫 杨莹莹 刘超群[1] 宋宁宁 杨彦伟 Zhang Shen;Feng Shan;Ma Longyu;Yang Yingying;Liu Chaoqun;Song Ningning;Yang Yanwei(Engineering Research Center for Nanomaterials,School of Pharmacy,Henan University,Kaifeng 475004;The First Affiliated Hospital of Henan University,Kaifeng 475100)

机构地区：[1]河南大学药学院纳米材料工程研究中心,开封475004 [2]河南大学第一附属医院,开封475100

出　　处：《化学学报》2022年第3期265-271,共7页Acta Chimica Sinica

基　　金：国家自然科学基金青年项目(No.52105180);河南省重点研发与推广项目(科技攻关)(No.212102210125);河南省高等学校重点科研项目(Nos.21A150013,22A350014);中国博士后科学基金面上项目(No.2021M690895)资助。

摘　　要：细菌感染是严重威胁着人们生命健康的主要问题之一.与传统抗生素疗法相比,新型抗菌策略光热疗法(PTT)展现出可控、微创及不易使细菌产生耐药性的优良性能.然而单模形式下PTT疗法并不理想,且高温下通常伴随着炎性反应等副作用,联合抗菌策略可有效解决这一问题.本研究制备了富含羧基的氧化介孔纳米碳球(OMCN),通过酰胺化反应,共价接枝聚六亚甲基双胍(PHMB),得到OMCN-PHMB纳米平台.实验结果表明, OMCN的光热性能具有良好的浓度与近红外光照功率依赖性.在808 nm激光照射下, OMCN-PHMB的体内外联合光热治疗效果显著优于单一模式下的其它治疗组,且组织学分析结果表明,该纳米平台对小鼠的主要器官没有产生明显的毒性,具有较高的生物相容性.Bacterial infection is one of the major problems that seriously threaten people’s life and health. In recent years,photothermal therapy(PTT), which uses photothermal conversion nanomaterials to convert optical energy into thermal energy for sterilization under specific wavelength laser irradiation, has aroused wide interest of researchers. Compared with traditional antibiotic therapy, the new anti-bacterial strategy photothermal therapy shows the excellent performance of controllable,minimally invasive and less bacterial resistance. However, monomodal PTT therapy is not ideal because it is often associated with side effects such as inflammatory reaction. Therefore, it is necessary to develop novel photothermal antibacterial system with high biocompatibility and safety to fight bacterial infection. Combined antibacterial strategy can effectively solve this problem. In this work, mesoporous carbon nanospheres(MCN) were prepared and oxidized by the mixed acid to obtain carboxyl-rich oxidized mesoporous carbon nanospheres(OMCN) with high biocompatibility and photothermal properties. Then, OMCNPHMB nano-antibacterial platform was obtained by grafting the antimicrobial agent poly(hexamethylene biguanide)hydrochloride(PHMB) onto the surface of OMCN with amide covalently. The photothermal properties of the system were evaluated and the results showed that the photothermal performance of OMCN had a good dependence on the concentration and power density of near-infrared light. Similar to the OMCN, the obtained OMCN-PHMB exhibited excellent performance of photothermal controllability and photothermal stability. In vivo and in vitro antibacterial experiments showed that the therapeutic effect of OMCN-PHMB under 808 nm laser irradiation was significantly better than that of other treatment groups under the single mode,which confirmed the excellent antibacterial effect of OMCN-PHMB combined with photothermal therapy. In addition, histological analysis showed that the nanoplatform had no significant toxicity to the major orga

关 键 词：细菌感染 光热疗法(PTT) 聚六亚甲基双胍(PHMB) 联合治疗 抗菌 

分 类 号：R318.08[医药卫生—生物医学工程]