益肾固本膏方对肾阳虚哮喘模型大鼠肾上腺皮质类固醇合成酶及糖皮质激素受体亚型的影响  

Effects of Yishen Guben Ointment on Adrenal Cortical Steroid Synthase and Glucocorticoid Receptor Subtypes in Rat Model with Kidney-yang Deficiency Pattern Asthma

作　　者：王云方[1] WANG Yunfang(Wuxi City Hospital of TCM,Wuxi 214000,China)

机构地区：[1]无锡市中医医院,江苏无锡214000

出　　处：《西部中医药》2022年第4期25-29,共5页Western Journal of Traditional Chinese Medicine

基　　金：无锡市卫计委卫生科研项目(Q201644)。

摘　　要：目的:研究益肾固本膏方对肾阳虚哮喘模型大鼠皮质类固醇合成酶11β-羟基类固醇脱氢酶1[hydroxysteroid(11-beta)dehydrogenase 1,HSD11B1)]、11β-羟化酶1(CYP11B1)及糖皮质激素受体亚型GRα、GRβ的影响。方法:将40只大鼠随机分为空白对照组,模型对照组,益肾固本膏方低、中、高剂量组,每组8只。建立肾阳虚哮喘大鼠模型,空白对照组与模型对照组以蒸馏水2 mL灌胃,益肾固本膏方各剂量组给予对应药物剂量灌胃,各组均灌胃20天。RT-PCR法测定大鼠肾上腺组织中HSD11B1、CYP11B1 mRNA及肺组织中GRα、GRβmRNA表达;Western Blot法测定大鼠肾上腺组织中HSD11B1、CYP11B1及肺组织中GRα、GRβ蛋白表达。结果:益肾固本膏方各剂量组HSD11B1、CYP11B1表达均高于模型组,差异有统计学意义(P<0.01)。益肾固本膏方各剂量组GRα表达均高于模型对照组(P<0.01),GRβ表达均低于模型对照组(P<0.01)。结论:益肾固本膏方可促进HSD11B1、CYP11B1合成,升高肾阳虚哮喘模型大鼠的血浆皮质酮水平,通过上调GRα含量、下调GRβ含量增强激素效应。Objective:To study the effects of Yishen Guben ointment(YSGB)on corticosteroid synthase 11β-hydroxysteroid dehydrogenase 1(HSD11B1),11β-hydroxylase 1(CYB11B1)and glucocorticoid receptor subtypes GRαand GRβin rats with kidney-yang deficiency pattern asthma.Methods:Forty rats were randomized into blank control group,model control group,low,moderate and high doses groups of YSGB,eight rats in each group.Rat models with kidney-yang deficiency pattern asthma were established,blank control group and model control group were perfused with distilled water,2mL,different doses groups of YSGB were drenched with the corresponding drug dose,all groups were administered for 20 days.To detect the expressions of HSD11B1,CYP11B1 mRNA and GRαand GRβmRNA in lung tissue by RT-PCR method;to measure the protein expression of HSD11B1,CYP11B1 in adrenal tissue and GRαand GRβprotein in lung tissue by Western Blot method.Results:The expressions of HSD11B1 and CYP11B1 in different doses groups of YSGB were higher than these of the model group,and the difference had statistical meaning(P<0.01).The expressions of GRαin different doses groups of YSGB were higher than these of model control group(P<0.01),the expressions of GRβlower than these of model control group(P<0.01).Conclusion:YSGB could raise the levels of plasma corticosterone in rat models with kidney-yang deficiency pattern asthma by promoting the synthesis of HSD11B1 and CYP11B1,it could enhance hormone effects by up regulating the contents of GRαand down regulating the contents of GRβ.

关 键 词：哮喘 肾阳虚证 益肾固本膏方 肾上腺皮质类固醇合成酶 糖皮质激素受体 11Β-羟基类固醇脱氢酶1 11β-羟化酶1 GRΑ GRΒ 动物实验 

分 类 号：R256.12[医药卫生—中医内科学]