基于熵权法-层次分析法结合响应面法优化养阴润目颗粒的提取工艺  

Optimization of Extraction Process for Yangyin Runmu Granules by Box-Behnken Design Based on Entropy Weight Method-analytic Hierarchy Process Method

作　　者：韩云凤 唐茜 石懿 蒲丽丽 潘琳 赖先荣[1] 张富文 HAN Yunfeng;TANG Xi;SHI Yi;PU Lili;PAN Lin;LAI Xianrong;ZHANG Fuwen(Traditional Chinese Medicine of Chengdu University,Chengdu 611137,China;Ophthalmic Hospital Affiliated to Chengdu University of Traditional Chinese Medicine,Chengdu 610081,China)

机构地区：[1]成都中医药大学,成都611137 [2]成都中医大银海眼科医院,成都610081

出　　处：《中国现代应用药学》2022年第7期896-903,共8页Chinese Journal of Modern Applied Pharmacy

基　　金：四川省科技计划项目(2017SZ0023)。

摘　　要：目的采用多指标综合加权评分法结合响应面法试验,优选养阴润目颗粒的最佳提取工艺。方法以HPLC测定葛根素、陈皮苷、异阿魏酸的含量,干膏得率及总多糖含量为综合评价指标,采用熵权法-层次分析混合加权法确定各评价指标的权重系数,通过单因素试验及响应曲面法分别考察加水量、提取次数、提取时间对养阴润目颗粒的水提工艺的影响,优选最佳提取工艺参数。结果按照混合加权法确定的各指标的客观权重进行综合评分,确定养阴润目颗粒的最佳提取工艺条件为加10倍量水,提取3次,每次65 min。验证试验结果显示预测值与实测值相比,RSD值≤3%。结论优选的提取工艺经验证,稳定、可行且重复性好,可为其后续养阴润目颗粒剂的开发研究提供实验参考。OBJECTIVE To optimize the optimal extraction process of Yangyin Runmu granules by using a multi-index comprehensive weighted scoring method combined with Box-Behnken design.METHODS Using the contents of puerarin,tangerine and isoferulic acid,ratio of dry extraction,total polysaccharides as comprehensive evaluation indicators,the weight coefficient of each evaluation index was determined by analytic entropy weight method-analytic hierarchy process.The single factor test and Box-Behnken design were used to investigate the influence of the water addition,time,and times of the water extraction process of Yangyin Runmu granules to optimize extraction process parameters.RESULTS According to the objective weight of each index determined by the mixed weighting method,the comprehensive scoring was performed,and the optimal extraction process condition of Yangyin Runmu granules was determined as adding 10 times of the amount of water,extracting 3 times,each time for 65 minutes.The verification test results show that compared with the measured value,the RSD of the predicted value was≤3%.CONCLUSION The optimized extraction process has been verified to be stable,feasible and reproducible,which can provide experimental reference for the subsequent development and research of Yangyin Runmu granules.

关 键 词：权重分析 熵权法-层次分析混合加权法 响应面法 总多糖 葛根素 陈皮苷 异阿魏酸 干膏率 

分 类 号：R284.2[医药卫生—中药学]