Identification of functionally active aerobic methanotrophs and their methane oxidation potential in sediments from the Shenhu Area,South China Sea  

作　　者：Jing Li Chang-ling Liu Neng-you Wu Xiao-qing Xu Gao-wei Hu Yan-long Li Qing-guo Meng 

机构地区：[1]Key Laboratory of Gas Hydrate,Ministry of Natural Resources,Qingdao Institute of Marine Geology,Qingdao 266071,China [2]Laboratory for Marine Mineral Resources,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266071,China [3]College of Marine Life Science,Ocean University of China,Qingdao 266100,China

出　　处：《China Geology》2022年第2期285-292,共8页中国地质（英文）

基　　金：jointly supported by the National Natural Science Foundation of China (42106052);Shandong Provincial Natural Science Foundation (ZR2020QD070);the project of China Geological Survey (DD20190221)。

摘　　要：Large amounts of gas hydrate are distributed in the northern slope of the South China Sea,which is a potential threat of methane leakage.Aerobic methane oxidation by methanotrophs,significant methane biotransformation that occurs in sediment surface and water column,can effectively reduce atmospheric emission of hydrate-decomposed methane.To identify active aerobic methanotrophs and their methane oxidation potential in sediments from the Shenhu Area in the South China Sea,multi-day enrichment incubations were conducted in this study.The results show that the methane oxidation rates in the studied sediments were 2.03‒2.36μmol/gdw/d,which were higher than those obtained by sediment incubations from other areas in marine ecosystems.Thus the authors suspect that the methane oxidation potential of methanotrophs was relatively higher in sediments from the Shenhu Area.After the incubations family Methylococcaea(type I methanotrophs)mainly consisted of genus Methylobacter and Methylococcaea_Other were predominant with an increased proportion of 70.3%,whereas Methylocaldum decreased simultaneously in the incubated sediments.Collectively,this study may help to gain a better understanding of the methane biotransformation in the Shenhu Area.

关 键 词：Active methanotrophs Aerobic methane oxidation Marine sediments Natural gas hydrates NGHs exploration trial engineering Oil and gas exploration engineering Shenhu Area South China Sea 

分 类 号：P618.13[天文地球—矿床学]